Publications
Annual & Progress Reports (July - June)
Patents
United States Patents (18)
Title: Production of chemicals from lignocellulose, biomass, or sugars.
Authors: K.T. Shanmugam, L.O. Ingram,M.A. Patel, M.S. Ou, R. Harbucker
Patent Number:7,098,009 (year approved 2006)
Title: Method and compositions for simultaneous saccharification and fermentation.
Authors:
L.O. Ingram, S. Zhou
Patent Number: 7,026,152 (2006)
Title:Ethanol production in recombinant hosts.
Authors: L.O. Ingram
Patent Number: 6,849,434(2005)
Title: Ethanol production from lignocellulose.
Authors: B.E. Wood, L.O. Ingram
Patent Number: 6,333,181(2001)
Title:Ethanol production using a soy hydrolysate- based medium or a yeast autolysate- based medium.
Authors: L.O. Ingram, S.W. York
Patent Number: 6,130,076 (2000)
Title: Recombinant cells that highly express chromosomally-integrated heterologous genes.
Authors: L.O. Ingram, K. Ohta, B.E. Wood
Patent Number: 6,107,093 (2000)
Title: Recombinant organisms capable of fermenting cellobiose.
Authors: L.O. Ingram, X. Lai,
M. Moniruzzaman, S.W. York
Patent Number: 6,102,690 (2000)
Title:Recombinant cells that highly express chromosomally-integrated heterologous genes.
Authors: L.O. Ingram, Kazuyoshi Ohta,
Brent E. Wood
Patent Number: 5,821,093 (1998);6,107,093(2000 )
Title: Recombinant glucose uptake system.
Authors: L.O. Ingram, Nico Arfman,
Jackie Snoep
Patent Number: 5,602,030 (1997)
Title: Ethanol production by gram-positive microbes.
Authors: L.O. Ingram,
M.D.F. Barbosa-Alleyne
Patent Number: 5,482,846 (1996); 5,916,787(1999)
Title:Ethanol production by recombinant hosts
Authors: LO. Ingram, DS. Beall,
GF. H. Burchhardt,
WV. Guimaraes, K. Ohta, B E. Wood,
KT. Shanmugam, D.E. Fowle,
A. Ben-Bassat
Patent Number: 5,424,202 (1995);5,487,989(1996); 5,424,202 (1995)
Title:Cloning and sequencing of the alcohol dehydrogenase II from Zymomonas mobilis.
Authors: L.O. Ingram, T. Conway
Patent Number: 5,162,516 (1992)
Title: Ethanol production using engineered mutant Escherichia coli
Authors: L.O. Ingram, D.C. Clark
Patent Number: 5,028,539 (1992)
Title: Ethanol production by Escherichia coli strains co-expressing Zymomonas pdc and adh genes.
Authors: L.O. Ingram, F. Alterthum,
T. Conway
Patent Number: 5,000,000 (1991)
International Patents (14)
Title:Ethanol production by genetically engineered Escherichia coli strains
Authors: L.O. Ingram, T. Conway, F. Alterthum
Country(Patent Number,Year Approved): Argentina(242634,1993); Canada(1335430,1995)
Title:Recombinant cells that highly express chromosomally- integrated heterologous genes.
Authors: L.O. Ingram, K. Ohta
Country(Patent Number,Year Approved): Australia(668677,1996); Europe(0560885,1999); Finland(111552,2003); Japan(3457664,2003)
Title: Ethanol production by recombinant hosts.
Authors: L.O. Ingram, D.S. Beall, G.F.H. Burchhardt, W.V. Guimaraes, K. Ohta, B.E. Wood, K.T. Shanmugam, D.E. Fowler, A. Ben-Bassat
Country(Patent Number,Year Approved): Australia(92672748,1997);Canada(2106377,2003); China(ZL92101877.0,2001); Europe(0576621,1992); Japan(3457664,2004); Mexico1(94048,1999)
Title: Recombinant microorganisms capable of fermenting cellobiose.
Authors: L.O. Ingram, X. Lai, M. Moniruzzaman, S.W. York
Country(Patent Number,Year Approved): Philippines (1-1998-00776,2003)
Title: Recombinant hosts suitable for simultaneous saccharification and fermentation.
Authors: L.O.ingram, S. Zhou
Country(Patent Number,Year Approved): New Zealand(516345,2004)
Additional National and International Patents Pending.
Book Chapters and Reviews (21)
Jarboe, L.R., K.T. Shanmugam, L.O. Ingram. Ethanol Encyclopedia of Microbiology (3rd Ed) Ed. D. Majumder-Russell. Elsevier, 2007 (in press)
Luli, G.W., L.R. Jarboe, L.O. Ingram. The Development of Ethanologenic Bacteria for Fuel Ethanol Production Bioenergy. Ed. A. Demain. ASM Press, 2007.
Jarboe, L.R., T.B. Grabar, L.P. Yomano, K.T. Shanmugam, L.O. Ingram. 2007. Development of ethanologenic bacteria. Book Chapter. Adv. Biochem Engin/Biotechnol 108: 237-261.
Ingram, L.O., H.C. Aldrich, A. Borges, A. Martinez, F. Martinez, A. Saleh, L.P.Yomano, S.W. York, J. Zaldivar, and S. Zou. 1999.. Enteric bacterial catalysts for fuel ethanol production. Biotechnol. Prog. 15: 855-866. [ABSTRACT]
Moniruzzaman, M. and L.O. Ingram. 1998. Ethanol production from lignocellulose using genetically engineered bacteria. Recent Res. Devel. Biotech. Bioeng. 1:259-269.
Ingram, L.O., P.F. Gomez, X. Lai, M. Moniruzzaman, B.E. Wood, L.P. Yomano, and S.W.York. 1998. Metabolic Engineering of bacteria for ethanol production. Biotechnology and Bioengineering 58:204-214. [ABSTRACT]
Ingram, L.O., X. Lai, M. Moniruzzaman, B.E. Wood, and S.W. York. 1997. Fuel ethanol production from lignocellulose using genetically engineered bacteria. In B.C. Saha and J. Woodward (eds.), Fuels and Chemicals from Biomass. ACS Symposium Series 666, p. 57-73. American Chemical Society Press, Washington, D.C.
Ingram. L.O. and J.B. Doran. 1995. Conversion of cellulosic materials to ethanol, FEMS Microbiol. Rev. 16: 235-241
Ingram, L.O., R.J. Bothast, J.B. Doran, D.S. Beall, T.A. Brooks, B.E. Wood, X. Lai, K. Asghari, and L.P. Yomano. 1995. Genetic Engineering of Bacteria for the conversion of lignocellulose to Ethanol. In J.I. Burke and B. Rice (eds.) Proceedings of the Workshop on Energy from Biomass and Waste, pp. 54-74. Teagasc, Oak Park Research Center, Carlow, Ireland.
Ingram, L.O. 1992. Genetic engineering of novel bacteria for the conversion of plant polysaccharides into ethanol. In M.R. Ladisch and A. Bose (ed), Harnessing Biotechnology for the 21st Century, p. 507-509. American Chemical Society Press, Washington, D.C.
Preston, J.F. , J.D. Rice, M.C. Chow and B.J. Brown.1991. Microbial strategies for the depolymerization of plant and algal polyuronides. In Enzymes in Biomass conversion. Eds. G.F. Leatham and M.E. Himmel, ACS Symposium Series 460:450-466.
Ingram, L.O. 1990. Ethanol tolerance in bacteria. Critical Rev. Biotechnology 9:305-319. [ABSTRACT]
Ingram, L.O., F. Alterthum, K. Ohta, and D.S. Beall. 1990. Genetic engineering of Escherichia coli and other enterobacteria for ethanol production,Developments in Industrial Microbiology, vol. 31, p. 21-30.
Ingram, L.O., K. Ohta, and D.S. Beall. 1990. Genetic modification of Escherichia coli for ethanol production, Energy from Biomass and Waste XIV (Don Klass, ed., Inst. Gas Technol. Chicago), p. 1105-1126.
Ingram L.O. and K.M. Dombek. 1989. Effects of ethanol on Escherichia coli. In N. van Uden (ed.), Alcohol Toxicity in Yeasts and Bacteria, CRC Press. p. 227-238.
Ingram, L.O.Y.A. Osman, T. Conway and K.M. Dombek. 1989. Zymomonas mobilis: Effects of ethanol on fermentation. In N. van Uden (ed.), Alcohol Toxicity in Yeasts and Bacteria, CRC Press. p. 257-270.
Ingram, L.O., C.K. Eddy, K.F. Mackenzie, T. Conway, and F. Alterthum. 1989. Genetics ofZymomonas mobilis and ethanol production. Developments in Industrial Microbiology vol. 30, p. 53-69.
Preston, J.F., A. Gibor, and M. Polne-Fuller. 1985. Manipulation of Sargassum for biomass production. In Methane from Biomass. A Systems Approach, J.R. Frank and W.H. Smith,eds. Elsevier pp. 211-234.
Ingram, L.O. and K.M. Dombek. 1987. On the evolution of alcohol tolerance in microorganisms. In Perspectives in Biotechnology (Duarte and Archer, eds.), p. 131- 138. Plenum Publishing Corp.
Ingram, L.O. 1986. Microbial tolerance to alcohols: Role of the cell membrane. Trends in Biotechnology 4:40-44.
Ingram, L.O., K.M. Dombek, and Y.A. Osman. 1986. A hypothesis for the Evolution of Microbial Resistance to Ethanol. Soc. Industr. Microbiol. News 36:8-11.
Ingram, L.O. and T.M. Buttke. 1984. Effects of alcohols on micro-organisms. Advances in Microbial Physiology 25: 253-300.
Journal Articles
Yomano, L.P., S.W. York, S. Zhou, K.T. Shanmugam, and L.O. Ingram. 2008. Re-engineering Escherichia coli B strain KO11 for ethanol production from xylose in mineral salts medium. Metabolic Engineering (submitted for publication).
170. Peterson, J.D., and L.O. Ingram. 2008. Anaerobic respiration in engineered Escherichia coli with an internal wlwxtron acceptor to produce fuel ethanol. Ann N.Y. Acad. Sci. 1125: 363-372.
169. Jantama, K., M.J. Haupt, S.A. Svoronos, X. Zhang, J.C. Moore, K.T. Shanmugam, L.O. Ingram. 2008. Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malate. Biotechnol and Bioengineering. 99(5): 1140-1153.
168. Shanmugan, K.T. and L.O. Ingram. 2008. Engineering biocatalysts for production of commodity chemicals. Journal of Molecular Microbiology and Biotechnology. 15:8-15.
167. Miller, E.N., L.O. Ingram. 2008. Sucrose and overexpression of trehalose biosynthetic genes (otsBA) increase dessication tolerance of recombinant Escherichia coli. Biotechnol Lett. 30: 503-508.
166. Rhee, M.S., J.-W. Kim, Y. Qian, L.O. Ingram, K.T. Shanmugam. 2007. Development of plasmid vector and electroporation condition for gene transfer in sporogenic lactic acid bacterium, Bacillus coagulans. Plasmid 58: 3-22.
165. Zhang, Xueli, K. Jantama, J.C. Moore, K.T.
Shanmugam and LO Ingram. 2007. Production of L-alanine by metabolically
engineered Escherichia coli in its current form. Appl Microbiol
Biotechnol. 77(2):355-66.
164. Kim, Y., L.O. Ingram, and K.T. Shanmugam. 2007.
Construction of an Escherichia coli K-12 for homoethanologenic
fermentation of glucose or xylose without foreign genes. Appl. and
Environ Microbiology. 17: 1766-1771.
163. Martinez, A., T.B. Grabar, K.T. Shanmugam, L.P. Yomano, S.W. York, and L.O. Ingram. 2007. Low salt medium for lactate and ethanol production by recombinant Esherichia coli B. Biotechnol. Lett. 29: 397-404.
162. Miller, E.N. and L.O. Ingram. 2006. Combined effect of betaine and trehalose on osmotic tolerance of Escherichia coli in mineral salts medium. Biotechnol. Lett. 29: 213-217.
161. Cirino, P.C., J.W. Chin and L.O. Ingram. 2006. Engineering Escherichia coli for xylitol production from glucose-xylose mixtures. Biotechnology and Bioengineering. 95:1167-1176.
160. Grabar, T. B. S. Zhou, K. T. Shanmugam, L. P. Yomano, & L. O. Ingram. 2006. Methylglyoxal bypass identified as source of chiral contamination in L(+) and D(-) lactate fermentations by recombinant Escherichia coli. Bioytechnology Letters 28:1527-1535.
159. Patel, M., M. Ou, L.O. Ingram, and K.T. Shanmugam. 2006. Isolation and charactrerization of acid-tolerant, thermophilic bacterial biocatalysts for effective fermentation of biomass-derived sugars to lactic acid. Appl and Environ. Microbiol. 72(5): 3228-3235.
158. Zhou, S., T.B. Grabar, K.T. Shanmugam and L.O. Ingram. 2006. Betaine tripled the volumetric productivity of D(-)-lactate by Escherichia coli strain SZ132 in mineral salts medium. Biotechnology Letters 28(9): 671-676.
157. Zhou, S., L.P. Yomano, K.T. Shanmugam, and L.O. Ingram. 2006. Fermentation of 10% (w/v) sugar to D(-)-lactate by engineered Escherichia coli B. Biotechnology Letters 28(9): 663-670.
156. Gutierrez, T., L.O. Ingram, J.F. Preston. 2006. Purification and characterization of a furfural reductase (FFR) from Escherichia coli strain LYO1- An enzyme important in the detoxification of furfural during ethanol production. J. of Biotechnology. 121(2): 154-164.
155. Talarico, L.A., M.A. Gil, L.O. Ingram, and J.A. Maupin-Furlow. 2005. Construction and expression of an ethanol production operon in gram-positive bacteria. Microbiology 151: 4023-4031.
154. Wood, B.E., L.P. Yomano, S.W. York, and L.O. Ingram. 2005. Development of industrial-medium-required elimination of the 2,3 butanediol fermentation pathway to maintain ethanol yield in an ethanologenic strain if Klebsiella oxytoca. Biotech. Prog.21: 1366-1372.
153. Tao, Han, Adnan Hasona, Phi Min Do, L.O. Ingram and K.T. Shanmugam. 2005. Global gene expression analysis revealed an unsuspected deo operon under the control of molybdate sensor, ModE protein, in Escherichia coli. Arch Microbiol: 184:225-233.
152. Patel MA, MS Ou , LO Ingram, and KT Shanmugam. 2005. Simultaneous saccharification and co-fermentation of crystalloine dellulose and sugar cane bagasse hemicellulose ahydrolysate to lactate by a thermotolerant acidophilic Bacillus sp. Biotechnology Progress 21(5): 1453-1460.
151. Ingram, LO. 2005. The potential role of alternative (renewable) fuels in the US. Journal of Biotechnology 118: S91-S91 Suppl.
150. Purvis J.E., L.P. Yomano, and L.O. Ingram. 2005. Enhanced trehalose production improves growth of Echerichia coli under osmotic stress. Appl. Envir. Microbiol. 2005 71: 3761-3769.
149. Hasona, A., Y. Kim, F.G. Healy, L.O. Ingram, and K.T. Shanmugam. 2004. Pyruvate formate lyase and acetate kinase are essential for anaerobic growth of Escherichia coli on xylose. J. Bacteriol. 186: 7593-7600.
148. Self, WT, Hasona, A., Shanmugam KT. 2004. Expression and regulation of a silent operon, hyf, coding for hydrogenase 4 isoenzyme in Escherichia coli. J Bacteriol. 2004 Jan;186(2):580-7.
147. Caccamo, M. A., Malone, C. S., and Rasche, M. E. 2004. Biochemical characterization of dihydromethanopterin reductase, a tetrahydromethanopterin biosynthesis enzyme in Methylobacterium extorquens AM1. J. Bacteriol. 186:2068-2073.
146. Rasche, M. E., Wyles, S. A., and Rosenzvaig, M. 2004. Characterization of two methanopterin biosynthesis mutants of Methylobacterium extorquens by use of a tetrahydromethanopterin bioassay. J. Bacteriol. 186:1565-1570.
145. Bobik, T.A., Rasche, M.E. 2004. Purification and partial characterization of the Pyrococcus horikoshii methylmalonyl-CoA epimerase. Applied Microbiology and Biotechnology 63:682-685.
144. Rasche, M. E. 2004. Outcomes of a research-driven laboratory and literature course designed to enhance undergraduate contributions to original research. Biochem. and Molecular Biology Educ. 32:101-107.
143. Kima, P.E., Rasche, M.E. 2004. Sex determination using the polymerase chain reaction. Biochem. and Molecular Biology Educ. 32:115-119.
142. Wang, X. Preston JF III, Romeo, T. 2004. The pgaABCD locus of Escherichia coli promotes the synthesis of a polysaccharide adhesin required for biofilm formation. J Bacteriol. 86(9):2724-34.
141. Maupin-Furlow, J. A., M. A. Gil, I. M. Karadzic, P. A. Kirkland, and C. J. Reuter. 2004. Proteasomes: perspectives from the archaea [update 2004]. Front Biosci. 9:1743-1758.
140. Reuter, C. J. and J. A. Maupin-Furlow. 2004. Analysis of proteasome-dependent proteolysis in Haloferax volcanii cells using short-lived green fluorescent proteins. Appl. Envir. Microbiol. 70:7530-7538.
139. Kaczowka, S. J., C. J. Reuter, L. A. Talarico and J. A. Maupin-Furlow. 2004. Recombinant production of Zymomonas mobilis pyruvate decarboxylase in the haloarchaeon Haloferax volcanii. Archaea 1(5).
138. Reuter, C. J., S. J. Kaczowka and J. A. Maupin-Furlow. 2004. Differential regulation of PanA and PanB proteasome-activating nucleotidase and 20S proteasome proteins of the haloarchaeon Haloferax volcanii. J. Bacteriol. 186:7763-7772.
137. Hasona, Adnan, Y. Kim, F.G. Healy, L.O. Ingram, and K.T. Shanmugam. 2004. Pyruvate formate lyase and acetate kinase are essential for anaerobic growth of Escherichia coli on xylose. J. Bacteriol. 186(22): 7593-7600.
136. Patel, M., M. Ou, L.O. Ingram, and K.T. Shanmugam. 2004. Fermentation of sugarcane bagasse hydrolysate to L(+) lactic acid by a thermotolerant acidophilic Bacillus sp. Biotechnology Letters. 26(11):865-8.
135. Maupin-Furlow, J. A., M. A. Gil, I. M. Karadzic, P. A. Kirkland, and C. J. Reuter. 2004. Proteasomes: perspectives from the archaea [update 2004]. Front Biosci. 9:1743-1758.
134. Underwood, S.A., M.L. Buszko, K.T. Shanmugam, and L.O. Ingram. 2004. Lack of protective osmolytes limits cell growth and volumetric productivity of ethanologenic Escherichia coli KO11 during xylose fermentation. Appl. & Environ. Microbiol. Appl. & Environ. Microbiology. 70(4): 2734-2740.
133. Shukla, V.B., S. Zhou, L.P. Yomano, K.T. Shanmugam, J.F. Preston, and L.O.Ingram. 2004. Production of D(-)-lactate from sucrose and molasses. Biotechnology Letters. 26: 689-693.
132. Caccamo MA, Malone CS, and Rasche ME. 2004. Biochemical characterization of a dihydromethanopterin reductase involved in tetrahydromethanopterin biosynthesis in Methylobacterium extorquens AM1. J. Bacteriol. 186:2068-2073.
131. Rasche ME, Havemann SA, and Rosentzvaig M. 2004. Characterization of two methanopterin biosynthesis mutants of Methylobacterium extorquens AM1 by use of a tetrahydromethanopterin bioassay. J. Bacteriol. 186:1565-1570.
130. Bobik TA., and Rasche ME. 2004. Purification and partial characterization of the Pyrococcus horikoshii methylmalonyl-CoA epimerase. Appl Microbiol Biotechnol. 63:682-685.
129. Maupin-Furlow, J. A., S. J. Kaczowka, C. J. Reuter, K. Zuobi-Hasona, and M. A. Gil. 2003. Archaeal proteasomes: potential in metabolic engineering. Metab. Eng. 5:151-163.
128. Kaczowka, S. J. and J. A. Maupin-Furlow. 2003. Subunit topology of the multiple 20S proteasomes from Haloferax volcanii. J. Bacteriol. 185:165-174.
127. Uz I, Rasche ME, Townsend T, Ogram AV, Lindner AS. 2003. Characterization of methanogenic and methanotrophic assemblages in landfill samples. Proc R Soc Lond B Biol Sci. 270 (Suppl. 2):S202-S205.
126. Dumitru R, Palencia. H, Schroeder SD, DeMontigny B, Takacs J., Rasche ME, Miner, JL, and Ragsdale, SW. 2003. Targeting methanopterin biosynthesis to inhibit methanogenesis. Appl. Environ. Microbiol. 69:7236-7241.
125. Bechard ME, Chhatwal S, Garcia RE, Rasche ME. 2003. Application of a colorimetric assay to identify putative RFAP synthase genes expressed with activity in Escherichia coli. Biological Procedures Online 5:69-77.
124. Bobik, TA, and Rasche, ME. 2003. HPLC assay for methylmalonyl-CoA epimerase. Anal. Bioanal. Chem. 375:344-349. Schmidt
123. Schmidt, L.M., J.F. Preston, D.W. Dickson, J.D. Rice, and T.E. Hewlett. 2003. Environmental quantification of Pasteuria penetrans endospores using in situ antigen extraction and immunodetection with a monoclonal antibody. FEMS Mocrobiol. Ecol. 44:17-26.
122. Preston, J. F., D. W. Dickson, J. E. Maruniak, G. Nong, J. A. Brito, L. M. Schmidt, and R. M. Giblin-Davis. 2003Pasteuria spp. : Systematics and phylogeny of these bacterial parasites of phytopathogenic nematodes. Journal of Nematology 35:198-207. Preston,
121.Preston, J.F., J.C. Hurlbert, J.D. Rice, A. Ragunathan, and F.J. St. John. 2003“Microbial Strategies for the Depolymerization of Glucuronoxylan: Leads to the Biotechnological Applications of Endoxylanases. ACS Symposium Ser 855, Application of Enzymes to Lignocellulosics. pp 191-210. Brito, J.
120. Brito, J.A., J. F. Preston, D. W.Dickson, R. M. Giblin-Davis, D. S. Williams, H. C. Aldrich, and J. D. Rice. Temporal Production and Immunolocalization of an Epitope During Pasteuria penetrans sporogenesis. Journal of Nematology 35(3):278-288 (2003).
119. Causey, T.B., K.T. Shanmugam, L.P. Yomano, and L.O. Ingram. 2003. Engineering Escherichia coli for efficient conversion of glucose to pyruvate. PNAS. 101(8): 2235-2240
118. Yilei, Qian, L.P. Yomano, J.F. Preston, H.C. Aldrich and L.O. Ingram. 2003. Cloning, characterization and functional expression of the Klebsiella oxytoca xyloside utilization operon (xynTB) in Escherichia coli. Applied and Environmental Microbiology. 69(10): 5957-5967.
117. Giblin-Davis, R.M., D. S. Williams, S. Bekal, D. W. Dickson, J. A. Brito, J. O. Becker and J. F. Preston. 2003 'Candidatus Pasteuria usgae' sp. nov., an obligate endoparasite of the phytoparasitic nematode Belonolaimus longicaudatus. Int J Syst Evol Microbiol 53: 197-200.
116. Gonzalez, R., H. Tao, J. E. Purvis, S. W. York, K. T. Shanmugam and L. O. Ingram.2003. Gene array-based identification of beneficial changes that contribute to increased ethanol tolerance in ethanologenic Escherichia coli LY01. Biotechnol. Prog. 19:612-623. [ABSTRACT]
115. Zhou, Shengde, K.T. Shanmugam and L.O. Ingram. 2003. Functional replacement of Escherichia coli D(-)-lactate dehydrogenase (ldhA) with L(+) -lactate dehydrogenase (ldhL) from Pediococcus acidilactici. Appl. Environ. Microbiol. 60(4): 2237-2244. [ABSTRACT]
114. Causey, T. B., S. Zhou, K. T. Shanmugam, and L. O. Ingram. 2003. Engineering the metabolism of Escherichia coli W3110 for the conversion of sugar to redox-neutral and oxidized products: homo-acetate production. Proc. Natl. Acad. Sci. USA. 100:825-832. [ABSTRACT]
113. Zhou, S., T.B. Causey, A. Hasona, K.T. Shanmugam, and L.O. Ingram. 2003. Production of optically pure D-lactic acid in mineral salts medium by metabolically engineered Escherichia coli W3110. Appl. Environ. Microbiol. 69(1):399-407. [ABSTRACT]
112. Scott JW, Rasche ME. 2002. Purification, overproduction, and partial characterization of beta-RFA-P synthase, a key enzyme in the pathway of methanopterin biosynthesis. J. Bacteriol. 184:4442-4448.
111. Hurlbert, J.C. and J.F. Preston. 2002. Differences in the solution structures of the parallel Beta-helical pectate lyases as determined by limited proteolysis. Biochimica et Biophysica Acta. 1599:9-20. [ABSTRACT]
110. Underwood, S.A.,S. Zhou, T. B. Causey, L.P. Yomano, K.T. Shanmugam, and L.O. Ingram. 2002. Genetic changes to optimize carbon partitioning between ethanol and biosynthesis ethanologenic Escherichia coli. Appl. Environ. Microbiol. 68(12): 6263-6272. [ABSTRACT]
109. Raj, K.C., L.A. Talarico, L.O. Ingram and J.A. Maupin-Furlow. 2002. Cloning and characterization of the Zymobacter palmae pyruvate decarboxylase (pdc): Comparison of bacterial homologoues. Appl. Environ. Microbiol. 68(6): 2869-2876. [ABSTRACT]
108. Gutierrez, T., M.L. Buszko, L.O. Ingram, and J.F. Preston. 2002. Reduction of furfural to furfuryl alcohol by ethanologenic strains of bacteria and its effect on ethanol production from xylose. Appl. Biochem. Biotechnol. 98-100: 327-340. [ABSTRACT]
107. Hasona, A., S.W. York, L.P. Yomano, L.O. Ingram and K.T. Shanmugam. 2002. Decreasing the level of ethyl acetate in ethanologenic fermentation broths of Escherichia coli KO11 by expression of Pseudomonas putida estZ esterase. Appl. Environ. Microbiol. 68(6): 2651-2659. [ABSTRACT]
106. Underwood, S.A., M.L. Buszko, K.T. Shanmugam, and L.O. Ingram. 2002. Flux through citrate synthase limits the growth of ethanologenic Escherichia coli KO11 during xylose fermentation. Appl. Environ. Microbiol. 68: 1071-1081. [ABSTRACT]
105. Gonzalez, R. Han Tao, K.T. Shanmugam, S. W. York, and L. O. Ingram. 2002. Global gene expression differences associated with changes in glycolytic flux and growth rate in Escherichia coli during the fermentation of glucose and xylose. Biotechnol. Prog. 18: 6-20. [ABSTRACT] [Gene Array Data]
104. Raj, K. C., L.O. Ingram and J. A. Maupin-Furlow. 2001. Pyruvate decarboxylase: a key enzyme for the oxidative metabolism of lactic acid by Acetobacter pasteurianus. Arch. Microbiol. 176: 443-451. [ABSTRACT]
103. Hurlbert, J.C., and J.F. Preston. 2001. Fuctional characterization of a novel xylanase from corn strains of Erwinia chrysanthemi. J. Bacteriol.183: 2093-2100. [ABSTRACT]
102. Hasona, A., Self, W.T. and K. T. Shanmugam. 2001. Transcriptional regulation of the moe (molybdate transport) operon of Escherichia coli. Arch. Microbiol. 175:178-188. [ABSTRACT]
101. Self, W. T., A. M. Grunden, A. Hasona and K. T. Shanmugam. 2001. Molybdate transport. Res. Microbiol. 152:311-321. [ABSTRACT]
100. Self, W. T., A. Hasona and K. T. Shanmugam. 2001. N-terminal truncations in the FhlA protein result in formate- and MoeA-independent expression of the hyc operon (formate hydrogenlyase) of Escherichia coli. Microbiology. 147:3093-3104. [ABSTRACT]
99. Talarico, L. A., L.O. Ingram and J.A. Maupin-Furlow. 2001. Production of Gram-positive Sarcina ventriculi pyruvate decarboxylase in Escherichia coli. Microbiology-SGM 147:2425-2433. [ABSTRACT]
98. Zhou, S. and L. O. Ingram. 2001. Simultaneous saccharification and fermentation of amorphous cellulose to ethanol by recombinant Klebsiella oxytoca SZ21 without supplemental cellulase. Biotechnol. Lett. 23: 1455-1462.
97. Han, T., R. Gonzales, A. Martinez, M. Rodriguez, L.O. Ingram, J.F. Preston, and K.T. Shanmugam. 2001. Engineering a homo-ethanol pathway in Eschericia coli: increased glycolytic flux and levels of expression of glycolytic genes during xylose fermentation. J. Bacteriol. 183: (10):2979-2988. [ABSTRACT] [Gene Array Data]
96. Martinez, A., M.E. Rodriguez, M.L. Wells, S.W. York, J.F. Preston, and L.O. Ingram. 2001. Detoxification of dilute acid hydrolysates of lignocellulose with lime. Biotechnol. Prog. 17: 287-293. [ABSTRACT]
95. Zhou, S., F.C. Davis, and L.O. Ingram. 2001. Gene integration and expression,a nd extracellular secretion of Erwinia chrysanthemi endoglucanase CelY (celY) and CelZ (celZ) in ethanologenic Klebsiella oxytoca P2. Appl. Environ. Microbiol. 67 (1) : 6-14. [ABSTRACT]
94. Self, W. T. and K. T. Shanmugam. 2000. Isolation and characterization of mutated FhlA proteins which activate transcription of hyc operon (formate hydrogenlyase) of Escherichia coli in the absence of molybdate. FEMS Microbiology Letters 184:47-52
93. Zhou, S. and L.O. Ingram. 2000. Synergistic hydrolysis of carboxymethyl cellulose and acid-swollen cellulose by two endoglucanases (EGY and EGZ) from Erwinia chrysanthemi. J. Bacteriol. 182: 5676-5682. [ABSTRACT]
92. Martinez, A., M.E. Rodriguez, S.W. York, J.F. Preston and L.O. Ingram. 2000. Use of UV absorbance to monitor furans in dilute acid hydrolysates of biomass. Biotechnol. Prog. 16: 637-641. [ABSTRACT]
91. Martinez, a., M.E. Rodriguez, S.W. York, J.F. Preston and L.O. Ingram. 2000. Effects of Ca(OH)2 treatments ("overliming")on the composition and toxicity of bagasse hemicellulose hydrolysates. Biotechnol. Bioengin. 69: 526-536. [ABSTRACT]
90. Zaldivar, J., A. Martinez and L.O. Ingram. 2000. Effect of alcohol components of hemicellulose hydrolysate on the growth and fermentation of ethanologenic Escherichia coli LY01. Biotechnol. Bioengin. 68: 524-530. [ABSTRACT]
89. Martinez-Morales, F., A.C. Borges, A. Martinez, K.T. Shanmugam and L.O. Ingram. 1999. Chromosomal integration of heterologous DNA in Escherichia coli with precise removal of markers and replicons used during construction. J. Bacteriol. 181: 7143-7148.
88. Zaldivar, J. and L.O. Ingram. 1999. Effect of organic acids on the growth and fermentation of ethanologenic Escherichia coli LY01. Biotechnol. Bioengin. 66: 203-210. Martinez, Alfredo, Sean W. York, Lorraine P. Yomano, Van L. Pineda, Francis C. Davis, John C. Shelton, and Lonnie O.
87. Ingram. 1999. Biosynthetic burden and plasmid burden limit expression of chromosomally integrated heterologous genes (pdc, adhB) in Escherichia coli. Biotechnol. Prog. 15: 891-897
86. Zhou, Shengde and Lonnie O. Ingram. 1999. Engineering endoglucanase-secreting strains of ethanologenic Klebsiella oxytoca P2. J. Industrial Microbiol. Biotechnol. 22: 600-607.
85. Zhou, Shengde, Lorraine P. Yomano, Alif. Z. Saleh, Francis C. Davis, Henry C. Aldrich and Lonnie O. Ingram. 1999. Enhancement of expression and apparent secretion of Erwinia chrysanthemi endoglucanase (encoded by celZ) in Escherichia coli B. Appl. Environ. Microbiol. 65:2439-2445. [ABSTRACT]
84. Zaldivar, Jesus, Alfredo Martinez and Lonnie O. Ingram. 1999. Effect of selected aldehydes on the growth and fermentation of ethanologenic Escherichia coli LY01 and KO11. Biotechnol. Bioengin. 65: 25-33.
83. Rasche, M. E., and White, R. H. (1998) Mechanism for the enzymatic formation of 4-( b-D-ribofuranosyl) aminobenzene 5'-phosphate during the biosynthesis of methanopterin. Biochemistry 37:11343-11351.
82. Moniruzzaman, M. and L.O. Ingram. 1998. Ethanol production from dilute acid hydrolysate of rice hulls using genetically engineered Escherichia coli. J. Industrial Microbiol. Biotechnol. 20:943-947.
81. Moniruzzaman, M., S.W. York, and L.O. Ingram. 1998. Effects of process errors on the production of ethanol by Escherichia coli KO11. J. Industrial. Microbiol. Biotechnol. 20:281-286.
80. Yomano, L.P., S.W. York, and L.O. Ingram. 1998. Isolation and characterization of ethanol tolerant mutants of Escherichia coli KO11 for fuel ethanol production. J. Industrial Microbiol. 20: 132-138. [ABSTRACT]
79. Wood, B.E., H.C. Aldrich, and L.O. Ingram. 1997. Ultrasound stimulates ethanol production during the simultaneous saccharification and fermentation of mixed waste office paper. Biotechnology Progress 13:232-237. [ABSTRACT]
78. Moniruzzaman, M., X. Lai, S.W. York, and L.O. Ingram. 1997. Isolation and molecular characterization of high-performance cellobiose-fermenting spontaneous mutants of ethanologenic Escherichia coli KO11 containing the Klebsiella oxytoca casAB operon. Appl. Environ. Microbiol. 63:4633-4637. [ABSTRACT]
77. Moniruzzaman, M., X. Lai, S.W. York, and L.O. Ingram. 1997. Extracellular melibiose and fructose are intermediates in raffinose catabolism during fermentation to ethanol by engineered enteric bacteria. J. Bacteriol. 179: 1880-1886. [ABSTRACT]
76. Wood, B.E., Beall, D.S. and L.O. Ingram. 1997. Production of recombinant bacterial endoglucanase as a co-product with ethanol during fermentation using derivatives of Escherichia coli KO11. Biotech. Bioengin. 55: 547-555.
75. Lai, X., F.C. Davis, R.B. Hespell, and L.O. Ingram. 1996. Cloning of cellobiose phosphoenolpyruvate-dependent phosphotransferase genes: Functional expression in recombinant Escherichia coli and identification of a putative binding region for disaccharides. Appl. Envir. Microbiol. 63:355-363. [ABSTRACT]
74. Dien, B.S., R.B. Hespell, L.O. Ingram, and R.J. Bothast. 1997. Conversion of corn milling fibrous co-products into ethanol by recombinant Escherichia coli strains KO11 and SL40. World J. Microbiology and Biotechnology 13: 619-625.
73. Moniruzzaman, M., B.S. Dien, B. Ferrer, R.B. Hespell, B.E. Dale, L.O. Ingram, and R.J. Bothast. 1996. Ethanol production from AFEX pretreated corn fiber by recombinant bacteria. Biotechnol. Lett. 18: 955-990.
72. York, S.W. and L.O. Ingram. 1996. Ethanol production by recombinant Escherichia coli KO11 using crude yeast autolysate as a nutrient supplement. Biotechnol. Lett. 18: 683-688.
71. York, S.W. and L.O. Ingram. 1996. Soy-based medium for ethanol production by Escherichia coli KO11. J. Indus. Microbiol. 16:374-376. [ABSTRACT]
70. Snoep, J.L., N. Arfman, L.P. Yomano, H.V. Westerhoff, T. Conway, and L.O. Ingram. 1996. Control of glycolytic flux in Zymomonas mobilis by glucose 6-phosphate dehydrogenase activity. Biotechnol. Bioengin. 51:190-197.
69. Asghari, A., R.J. Bothast, J.B. Doran, and L.O. Ingram. 1996. Ethanol production from hemicellulose hydrolysates of agricultural residues using genetically engineered Escherichia coli strain KO11. J. Industrial Microbiol. 16: 42-47.
68. Brooks, T.A., and L.O. Ingram. 1995. Conversion of mixed waste office paper to ethanol by genetically engineered Klebsiella oxytoca strain P2. Biotechnology Progress. 11:619-625.
67. Snoep, J.L., L.P. Yomano, H.V. Westerhoff, and L.O. Ingram. 1995. Protein burden in Zymomonas mobilis: Negative flux and growth control due to overproduction of glycolytic enzymes. Microbiology. 141:2329-2337.
66. Parker, C., W.O. Barnell, J.L. Snoep, L.O. Ingram and T. Conway. 1995. Characterization of the Zymomonas mobilis glucose facilitator gene product (glf) in recombinant Escherichia coli: examination of transport mechanism, kinetics and the role of glucokinase in glucose transport. Mol. Microbiol. 15:795-802. [ABSTRACT]
65. Lai, X. and L.O. Ingram. 1995. Discovery of a ptsHI operon, which includes a third gene (ptsT), in the thermophile Bacillus stearothermophilus. Microbiology. 141:1443-1449. [ABSTRACT]
64. Healey, F.G., R.M. Ray, H.C. Aldrich, A.C. Wilkie, L.O. Ingram, and K.T. Shanmugam. 1995. Direct isolation of functional genes encoding cellulases from the microbial consortia in a thermophilic, anaerobic digester maintained on lignocellulose. Appl. Microbiol Biotechnol. 43:667-674. [ABSTRACT]
63. Lindsay, S.E., R.J. Bothast, and L.O. Ingram. 1995. Improved strains of recombinant Escherichia coli for ethanol production from sugar mixtures. Appl. Microbiol. Biotechnol. 43:70-75. [ABSTRACT]
62. Gomez, P.F. and L.O. Ingram. 1995. Cloning, sequencing, and characterization of the alkaline phosphatase gene (phoD) from Zymomonas mobilis. FEMS Letters. 125:237-246. [ABSTRACT]
61. Barbosa, M. de F.S., L.P. Yomano and L.O. Ingram. 1994. Cloning, sequencing, and expression of the stress genes from the ethanol-producing bacterium Zymomonas mobilis: The groESL operon. Gene 148:51-57. [ABSTRACT]
60. Bothast, R.J., B.C. Saha, A.V. Flosenzier, and L.O. Ingram. 1994. Fermentation of L-arabinose, D-xylose and D- glucose by ethanologenic recombinant Klebsiella oxytoca strain P2. Biotechnology Letters 16:401-406.
59. Doran, J.B., H.C. Aldrich, and L.O. Ingram. 1994. Saccharification and fermentation of sugar cane bagasse by Klebsiella oxytoca P2 containing chromosomally integrated genes encoding the Zymomonas mobilis ethanol pathway. Biotechnology and Bioengineering 44:240-247.
58. Grohmann, K., E.A. Baldwin, B.S. Buslig, and L.O. Ingram. 1994. Fermentation of galacturonic acid and other sugars in orange peel hydrolysates by the ethanologenic strain of Escherichia coli. Biotechnology Letters 16:281-286.
57. Snoep, J.L., N. Arfman, L.P. Yomano, R.K. Fliege, T. Conway, and L.O. Ingram. 1994. Reconstitution of glucose uptake and phosphorylation in a glucose negative mutant of Escherichia coli using Zymomonas mobilis genes encoding the glucose facilitator protein and glucokinase. J. Bacteriol. 176:2133-2135. [ABSTRACT]
56. Barbosa, M. de F.S. and L.O. Ingram. 1994. Expression of the Zymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes in Bacillus. Current Microbiology 28:279-282.
55. Lai, X. and L.O. Ingram. 1993. Cloning and sequencing of a cellobiose phosphotransferase system operon from Bacillus stearothermophilus XL-65-6 and functional expression in Escherichia coli. J. Bacteriol. 175: 6441-6450. [ABSTRACT]
54. Doran, J.B. and L.O. Ingram. 1993. Fermentation of crystalline cellulose to ethanol by Klebsiella oxytoca containing chromosomally integrated Zymomonas mobilis genes. Biotechnology Progress 9:533-538.
53. Zhao, G., T. Xia, L.O. Ingram, and R.A. Jensen. 1993. An allosterically insensitive class of cyclohexadienyl dehydrogenase from Zymomonas mobilis. Eur. J. Biochem. 212:157-165. [ABSTRACT]
52. Yomano, L.P., R.K. Scopes, and L.O. Ingram. 1993. Cloning, sequencing, and expression of the Zymomonas mobilis phosphoglycerate mutase gene (pgm) in Escherichia coli. J. Bacteriol. 175: 3926-3933. [ABSTRACT]
51. Burchhardt, G., K.F. Keshav, L. Yomano, and L.O. Ingram. 1993. Mutational analysis of segmental stabilization of transcripts from the Zymomonas mobilis gap-pgk operon. J. Bacteriol. 175:2327-2333. [ABSTRACT]
50. Beall, D.S. and L.O. Ingram. 1993. Genetic engineering of soft-rot bacteria for ethanol production from lignocellulose. J. Industrial Microbiol. 11:151-155.
49. Beall, D.S., L.O. Ingram, A. Ben-Bassat, J.B. Doran, D.E. Fowler, R.G. Hall, and B.E. Wood. 1992. Conversion of hydrolysates of corn cobs and hulls into ethanol by recombinant Escherichia coli B containing integrated genes for ethanol production. Biotechnology Letters 14: 857-862.
48. Arfman, N., V. Worrell, and L.O. Ingram. 1992. Use of the tac promoter and lacIq for the controlled expression of Zymomonas mobilis fermentative genes in E. coli and Z. mobilis. J. Bacteriol. 174: 7370-7378. [ABSTRACT]
47. Aldrich, H.C., L. McDowell, M. de F.S. Barbosa, L. Yomano, R.K. Scopes, and L.O. Ingram. 1992. Immunocytochemical localization of glycolytic and fermentative enzymes in Zymomonas mobilis. J. Bacteriol. 174: 4504-4508. [ABSTRACT]
46. Mejia, J.P., M.E. Burnett, H. Ann, W.O. Barnell, K.F. Keshav, T. Conway, and L.O. Ingram. 1992. Coordination of expression of Zymomonas mobilis glycolytic and fermentative enzymes: A simple hypothesis based on mRNA stability. J. Bacteriol. 174:6438-6443. [ABSTRACT]
45. Wood, B.E. and L.O. Ingram. 1992. Ethanol production from cellobiose, amorphous cellulose, and crystalline cellulose by recombinant Klebsiella oxytoca containing chromosomally integrated Zymomonas mobilis genes for ethanol production and plasmids expressing thermostable cellulase genes from Clostridium thermocellum. Appl. Environ. Microbiol. 58: 2103-2110. [ABSTRACT]
44. Guimaraes, W.V., K. Ohta, G. Burchhardt, and L.O. Ingram. 1992. Ethanol production from starch by recombinant Escherichia coli containing integrated genes for ethanol production and plasmid genes for saccharification. Biotechnology Letters 14:415-420.
43. Burchhardt, G., and L.O. Ingram. 1992. Conversion of xylan to ethanol by ethanologenic strains of Escherichia coli and Klebsiella oxytoca. Appl. Environ. Microbiol. 58:1128-1133. [ABSTRACT]
42. Guimaraes, W.V., G.L. Dudey, and L.O. Ingram. 1992. Fermentation of sweet whey by ethanologenic Escherichia coli. Biotechnology and Bioengineering 40:41-45.
41. Preston III, James F., John D. Rice, Lonnie O. Ingram, and Noel T. Keen. 1992. Differential depolymerization mechanisms of pectate lyases secreted by Erwinia chrysanthemi EC 16. J. Bacteriol. 174:2039-2042. [ABSTRACT]
40. Barbosa, M. de F.S., M.J. Beck, J.E. Fein, D. Potts, and L.O. Ingram. 1992. Efficient fermentation of Pinus sp. acid hydrolysates by an ethanologenic strain of Escherichia coli. Appl. Environ. Microbiol. 58:1382-1384. [ABSTRACT]
39. Brown, B.J., J.F. Preston, and L.O. Ingram. 1991. Cloning of alginate lyase gene (alxM) and expression in Escherichia coli. Appl. Environ. Microbiol. 57:1870-1872. [ABSTRACT]
38. Ohta, K., D.S. Beall, J.P. Mejia, K.T. Shanmugam, and L.O. Ingram. 1991. Metabolic engineering of Klebsiella oxytoca M5A1 for ethanol production from xylose and glucose. Appl. Environ. Microbiol. 57:2810-2815. [ABSTRACT]
37. An, Haejung, R.K. Scopes, M. Rodriguez, and L.O. Ingram. 1991. Gel electrophoretic analysis of Zymomonas mobilis glycolytic and fermentative enzymes: identification of alcohol dehydrogenase II as a stress protein. J. Bacteriol. 173:5975-5982. [ABSTRACT]
36. Beall, D.S., K. Ohta, and L.O. Ingram. 1991. Parametric studies of ethanol production from xylose and other sugars by recombinant Escherichia coli. Biotechnology and Bioengineering 38:296-303.
35. Utt, E.A., C.K. Eddy, K.F. Keshav, and L.O. Ingram. 1991. Sequencing and expression of the Butyrivibrio fibrisolvens xylB gene encoding a novel bifunctional protein with beta-D-xylopyranosidase and alpha-L- rabinofuranosidase activities. Appl. Environ. Microbiol. 57:1227-1234. [ABSTRACT]
34. Ohta, Kazuyoshi, D.S. Beall, K.T. Shanmugam, and L.O. Ingram. 1991. Genetic improvement of Escherichia coli for ethanol production: chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase and alcohol dehydrogenase II. Appl. Environ. Microbiol. 57:893-900. [ABSTRACT]
33. Eddy, C.K., K.F. Keshav, Haejung An, E.A. Utt, J.P. Mejia, and L.O. Ingram. 1991. Segmental message stabilization as a mechanism for differential expression from the Zymomonas mobilis gap operon. J. Bacteriol. 173:245-254. [ABSTRACT]
32. Keshav, K.F., L.P. Yomano, Haejung An, and L.O. Ingram. 1990. Cloning of the Zymomonas mobilis structural gene encoding alcohol dehydrogenase I (adhA): Sequence comparison and expression in Escherichia coli. J. Bacteriol. 172: 2491-2497. [ABSTRACT]
31. Ohta, K., F. Alterthum, and L.O. Ingram. 1990. Effects of environmental conditions on xylose fermentation by recombinant Escherichia coli. Appl. Environ. Microbiol. 56:463-465. [ABSTRACT]
30. Eddy, C.K., J.P. Mejia, T. Conway, and L.O. Ingram. 1989. Differential expression of gap and pgk genes within the gap operon of Zymomonas mobilis. J. Bacteriol. 171:6549-6554. [ABSTRACT]
29. Conway, T., and L.O. Ingram. 1989. Similarity of Escherichia coli propanediol oxidoreductase (fucO product) and an unusual alcohol dehydrogenase from Zymomonas mobilis and Saccharomyces cerevisiae. J. Bacteriol. 171:3754-3759. [ABSTRACT]
28. Mackenzie, Kylie F., T. Conway, H.C. Aldrich, and L.O. Ingram. 1989. Expression of Zymomonas mobilis adhB encoding alcohol dehydrogenase II and adhB- acZ operon fusions in recombinant Zymomonas mobilis. J. Bacteriol. 171: 4577-4582. [ABSTRACT]
27. Alterthum, Flavio, and L.O. Ingram. 1989. Efficient ethanol production from glucose, lactose, and xylose by recombinant Escherichia coli. Appl. Environ. Microbiol. 55:1943-1948. [ABSTRACT]
26. Alterthum, Flavio, K.M. Dombek, and L.O. Ingram. 1989. Regulation of glycolytic flux and ethanol production in Saccharomyces cerevisiae: Effects of intracellular adenine nucleotide concentrations on the in vitro activities of hexokinase, phosphofructokinase, phosphoglycerate kinase, and pyruvate kinase. Appl. Environ. Microbiol. 55:1312-1314.
25. Mackenzie, K.F., C.K. Eddy, and L.O. Ingram. 1989. Modulation of alcohol dehydrogenase isoenzyme levels in Zymomonas mobilis by iron and zinc. J. Bacteriol. 171: 1063-1067. [ABSTRACT]
24. Sewell, G.W., E.A. Utt, R.B. Hespell, K.F. Mackenzie, and L.O. Ingram. 1989. Identification of the Butyrivibrio fibrisolvens xylosidase gene (xylB) coding region and its expression in Escherichia coli. Appl. Environ. Microbiol. 55: 306-311. [ABSTRACT]
23. Pond, J.L., C.K. Eddy, Kylie F. Mackenzie, T. Conway, D.J. Borecky, and L.O. Ingram. 1989. Cloning, sequencing, and characterization of the principal acid phosphatase, the phoC product, from Zymomonas mobilis. J. Bacteriol. 171: 767-774. [ABSTRACT]
22. Sewell, G.W., H.C. Aldrich, D. Williams, B. Mannerelli, A. Wilkie, R.B. Hespell, P.H. Smith, and L.O. Ingram. 1988. Isolation and characterization of xylan-degrading strains of Butyrivibrio fibrisolvens from a napiergrass-fed anaerobic digester. Appl. Environ. Microbiol. 54: 1085-1090.
21. Conway. T. and L.O. Ingram. 1988. Phosphoglycerate kinase gene from Zymomonas mobilis: Cloning, sequencing and localization within the gap operon. J. Bacteriol. 170:1926-1933. [ABSTRACT]
20. Ingram, L.O. and T. Conway. 1988. Expression of different levels of ethanologenic enzymes from Zymomonas mobilis in recombinant strains of Escherichia coli. Appl. Environ. Microbiol. 54: 397-404.
19. Dombek, K.M. and L.O. Ingram. 1988. Intracellular accumulation of AMP as a cause for the decline in rate of ethanol production by Saccharomyces cerevisiae during batch fermentation. Appl. Environ. Microbiol. 54:98-104.
18. Conway, T., G.W. Sewell and L.O. Ingram. 1987. Glyceraldehyde-3-phosphate dehydrogenase gene from Zymomonas mobilis (gap): Cloning, sequencing, and identification of promoter region. J. Bacteriol. 169:5653-5662. [ABSTRACT]
17. Ingram, L.O., T. Conway, D.P. Clark, G.W. Sewell, and J.F. Preston. 1987. Genetic engineering of ethanol production in Escherichia coli. Appl. Environ. Microbiol. 53:2420-2425. [ABSTRACT]
16. Osman, Y.A., T. Conway, S.J. Bonetti, and L.O. Ingram. 1987. Glycolytic flux in Z. mobilis: Enzyme and metabolite levels during batch fermentation. J. Bacteriol. 169:3726-3736. [ABSTRACT]
15. Osman, Y.A. and L.O. Ingram. 1987. Zymomonas mobilis mutants with an increased rate of alcohol production. Appl. Environ. Microbiol. 53:1425-1432.
14. Conway. T., Y.A. Osman and L.O. Ingram. 1987. Gene expression in Zymomonas mobilis: Promoter structure and identification of membrane anchor sequences forming functional LacZ' Fusion proteins. J. Bacteriol. 169:2327-2335. [ABSTRACT]
13. Conway, T.C., G.W. Sewell, Y.A. Osman and L.O. Ingram. 1987. Cloning and sequencing of the alcohol dehydrogenase II from Zymomonas mobilis. J. Bacteriol. 169:2591-2597. [ABSTRACT]
12. Dombek, K.M. and L.O. Ingram. 1987. Ethanol production during batch fermentation with Saccharomyces cerevisiae: Changes in glycolytic enzymes and internal pH. Appl. Environ. Microbiol. 53:1286-1291. [ABSTRACT]
11. Conway, T., Y.A. Osman and L.O. Ingram. 1987. Promoter and nucleotide sequence of the Zymomonas mobilis pyruvate decarboxylase. J. Bacteriol. 169:949-954. [ABSTRACT]
10. Dombek, K.M. and L.O. Ingram. 1986. Magnesium limitation and its role in the apparent toxicity of ethanol during yeast fermentation. Appl. Environ. Microbiol. 52:975-981. [ABSTRACT]
9. Byun, M. O.-K., J.B. Kaper and L.O. Ingram. 1986. Construction of a new vector for the expression of foreign genes in Zymomonas mobilis. Journal of Industrial Microbiology 1:9-15.
8. Domek, K.M. and L.O. Ingram. 1986. Determination of the intracellular concentration of ethanol in Saccharomyces cerevisiae during fermentation. Appl. Environ. Microbiol. 51:197-200. [ABSTRACT]
7. Osman, Y.A. and L.O. Ingram. 1985. Mechanism of ethanol inhibition of fermentation in Zymomonas mobilis strain CP4. J. Bacteriol. 164:173-180. [ABSTRACT]
6. Ingram, L.O., A.S. Holt, V.C. Carey, K.M. Dombek, W.A. Holt, Y.A.Osman and S.K. Walia. 1984. Biochemical and Genetic Improvement of Zymomonas mobilis. Biomass. 6:131-143.
5. Carey, V.C., S.K. Walia and L.O. Ingram. 1984. Expression of the lactose operon in Zymomonas mobilis. Devel. Industr. Microbiol. 25:589-596.
4. Carey, V.C., S.K. Walia and L.O. Ingram. 1983. Expression of a lactose transposon (TN951) in Zymomonas mobilis. Appl. Environ. Microbiol. 46:1163-1168.
3. Carey, V.C. and L.O. Ingram. 1983. Lipid composition of Zymomonas mobilis: Effects of ethanol and glucose. J. Bacteriol. 154:1291-1300. [ABSTRACT]
2. Eaton, L.C., T.F. Tedder and L.O. Ingram. 1982. Effects of fatty acid composition on the sensitivity of membrane functions to ethanol in Escherichia coli. Substance and Alcohol Actions/Misuse 3:77-87. [ABSTRACT]
1. Ingram, L.O., N.S. Vreeland and L.C. Eaton. 1980. Alcohol tolerance in Escherichia coli. Pharmacol. Biochem. Behavior 13 (suppl. 1) 13:191-195. [ABSTRACT]
