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Progress Report
The
Florida Center for Renewable
July 2001- June 2003
University of Florida
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CONTACT INFORMATION
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TABLE OF CONTENTS
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Introduction
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Faculty Member/Visiting Scientist |
Place and Date |
Topic |
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James F. Preston, III |
Department of Microbiology and Cell Science, September 3, 2001. |
“Pasteuria Spp.: Endospore-Forming Bacterial Parasites Of Cladocerans And Phytopathogenic Nematodes”. |
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Department of Plant Pathology, January 14, 2003. |
“Pasteuria Spp.: Endospore-Forming Bacterial Parasites Of Cladocerans And Phytopathogenic Nematodes As Naturally Occurring Biocontrol Agents.” |
In-house seminars are conducted during the Fall and Spring Semesters. Research progress reports by undergraduate, and graduate students, post-docs, staff members, and research faculty members are presented for discussion and suggestions. Twenty five to thirty people regularly attend this weekly seminar.
Faculty members and their students attended conferences and seminars to present research results and to interact with other researchers from other academic and industrial institutions. The Table in the following pages shows the conference, seminar, or workshop, place and date, and topics presented by attendees.
Table. 1. Conferences, Workshops, and Meetings Attended by
Members of the FCRC.
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Conference/Seminar/ |
Date and Place |
Attendees/Poster Presenters/Speakers |
Topic |
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Symposium for the Society of Nematology. |
Salt Lake City, 2001 |
Preston, J.F., D.W. Dickson, J.E. Maruniak, J.A. Brito, L.M. Schmidt, and J.M. Anderson |
Pasteuria Spp.: Systematics-Phylogeny Of These Unusual Bacterial Parasites Of Phytopathogenic Nematodes. |
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Conference on Interfacing Microbiology and Biotechnology |
University of Florida, Gainesville, FL May 17-19, 2001. |
J.F. Preston, III |
Microbial Strategies For The Depolymerization Of Glucuronoxylan. |
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Madeline E. Rache |
C1 Metabolism And Methanogens |
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Lonnie O. Ingram and James F. Preston, III |
Metabolic Engineering And Regulation |
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K.T. Shanmugam |
Bioinformatics And Global Gene Expression |
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Julie A. Maupin-Furlow |
Nanocompartments Of Prokaryotic Cells |
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101st American Society for Microbiology General Meeting |
Orlando, FL. May 20-24, 2001. |
Lonnie O. Ingram
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Symposium Speaker: Genetic Engineering For Production Of Industrial Chemicals. |
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Rebecca M. Harbucker* |
Isolation Of A Thermotolerant Acid Resistant Host For The adh And pdc Genes Of Zymomonas mobilis To Enable Cost Effective Production Of Ethanol For Biomass. |
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ME Rodriguez* |
Properties Of The Hemicellulose Fractions Of Lignocellulosic Biomass Affecting Bacterial Ethanol Production. |
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Tony Gutierrez* |
Biotransformation Of Furfural By Ethanologenic Bacteria And Its Effect On Ethanol Production. |
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Lee Ann Talarico* |
Cloning And Characterization Of Pyruvate Decarboxylase From SarcinavVentriculi. |
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FrankG. Healy* |
Development Of Ethanologenic Escherichia coli For The Simultaneous Utilization Of Glucose And Xylose. |
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Stuart A. Underwood* |
Additional Osmoprotectants Are Required For The Efficient Fermentation Of 10% Xylose To Ethanol In Nutrient-Limiting Media |
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Ramon Gonzalez* |
Identification Of Promoters For Programmed Expression Of Recombinant Products Im Escherichia Coli By Transcriptone Analysis. |
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Steven J. Kaczowka* |
Structure And Function Of Proteasomes From A Halophilic Archaeon Halofreax volcanii. |
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H.L. Wilson* |
Purification And Characterization Of The 20S Proteasome And PAN From Methanococcus maripaludis. |
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Mark S. Ou* |
Influence Of The Alpha Subunit On The Proteolytic Activity Of 20S Proteosomes |
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Kheir Zuobi-Hasona** |
Oligosaccharides Containing Glucuronoxylose As Substrates For Selecting Bacteria For Depolymerization Of Hemicellulose. |
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Shengde Zhou |
Fermentation Of Cellulooligosaccharides And Amorphous Cellulose To Ethanol By Cellulolytic Detivatives Of Klebsiella oxytoca. |
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A.M. Paredes, F. Soyer, H.C. Aldrich, S. Lutke, H Tsuruta, W.Chiu, JM Shively |
Three-dimensional structure of Thiobacilllus neapolitanus Carboxysomes as Determined by Cryo-electon Microscopy. |
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E.M. Sampson, G.D. Haveman, H.C. Aldrich, T.A. Bobik |
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Gordon Research Conference on Archaea: Ecology, Metabolism, and Molecular Biology |
2001 Proctor Academy, NH |
Steven Kaczowka* |
Localization Of The Alpha-1 And Alpha-2 Subunits Of 20S Proteasomes From A Halophilic Archaeon, Haloferax volcanii’ |
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2nd Gordon Conference on Molybdenum and Tungsten Enzymes |
Queen’s College, Oxford, England July 1-6, 2001 |
K.T. Shanmugam |
Molybdenum Metabolism. |
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University of Florida, Dept. of Microbiology and Cell Science |
Gainesville, FL, 2001 |
Julie A. Maupin-Furlow |
Energy-dependent Proteolysis in the Archaea. |
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Gordon Research Conference, Archaea: Ecology, metabolism, and Microbiology |
Andover, NH, 2002 |
Julie A. Maupin-Furlow |
Archael Proteosomes and Proteosome-Activating Nucleotidases. |
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Brookhaven National Laboratories |
Upton, NY, 2001 |
Julie A. Maupin-Furlow |
Proteosomes: Molecular Machines Integral to the Genomes to Life Initiative. |
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223rd National Meeting of the American Chemical Society. |
Orlando, FL April 7-11, 2002. |
Preston, J.F., and J.C. Hurlbert |
Microbial Strategies For The Depolymerization Of Methylglucuronoxylan.- Payen Award Symposium Honoring Liisa Viikari: Application Of Enzymes To Lignocellulosics. |
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23rd Symposium on Biotechnology for Fuels and Chemicals |
Breckenridge, Colorado May 4-7, 2003 |
Stuart Underwood* |
Optimizing Carbon Partitioning between Biosynthesis and Ethanol Production in Ethanologenic Escherichia coli KO11 |
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James F. Preston, Franz J. St. John and John D. Rice |
Selection of Endoxylanases for Depolymerization of Glucuronoxylan from Hemicellulose |
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Annual Meeting of the American Society for Microbiology Southeastern Branch |
Gainesville, Florida, on November 7-9, 2002 |
Lonnie O. Ingram |
Engineering E. coli For Renewable Chemicals And Fuels. |
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K.T. Shanmugam |
Whole Genome Expression Analysis Of E. coli Growing Under Aerobic And Anaerobic Condition. |
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Bryce Causey* |
Microbial Platform For The Production Of Oxidized Compounds (Acetate And Pyruvate) From Escherichia coli W3110 ATCC. |
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Lee Ann Talarico* |
Characterization Of Pyruvate Decarboxylase From Sarcina ventriculi. |
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Franz St. John* |
Xylan Utilization By Paenibacillus Sp Secresting a GH10 Xylanase With Significant Homology To GH10 Of Clostridium josui. |
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Stuart Underwood* |
Balancing Carbon Partitioning Between Biosynthesis And Product Formation |
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Shengde Zhou |
Metabolic Engineering Escherichia coli W3110 Fro Production Of Optically Pure D(-) And L(+)-Lactic Acid From Mineral Salts Medium. |
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Christopher Reuter* |
Biochemical Characterization And Subunit Arrangement Of PAN Proteins From Halophilic Archeon Haloferax volcanii. |
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Steven J. Kaczowka* |
Subunit Arrangement Of 20S Proteosomes From Haloferax volanii. |
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Phoung-Uyen Le*** |
Thermostability Of Sarcina ventriculi Pyruvate Decarboxylase At Ph 5.5 |
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Brea Duval*** |
Generation Of Coupled Microtiter Plat Assay For Sarcina ventriculi Pyruvate Decarboxylase. |
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Cheryl Boice* |
Characterization Of Bacteriphages For Use In Adsorption And Transport Studies. |
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Johnny Davis* |
Fate Of Bacterial Indicators And Pathogens In Anaerobically Digested Dairy Wastewater. |
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Yilei Qian |
Isolation Of The Xylosidase Gene (Xynb) Operon From Klebsiella oxytoca. |
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Liesbeth Schmidt** |
Detection Of Pasteuria penetrans P20 Infected Meloidogyne arenaria Race 1 By Polymerase Chain Reaction (PCR). |
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Vilas Shukla** |
Engineering Escherichia coli For Lactic Acid Production From Sucrose. |
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Brent Wood* |
Reprioritization Of Sugar Utilization Profiles By Overexpression Of A CRP* In Escherichia coli KO11 |
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Annual Meeting of the American Society for Microbiology |
Salt Lake City, Utah, 2002 |
Julie A. Maupin-Furlow |
Compartmentalization of Proteolysis Mediated by the Archaeal Proteosome. |
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G.C. Cannon, S. Heinhorst, H.C. Aldrich, E. Williams, J. Shively |
Characterization of Halothiobacillus neapolotanus Carboxysomes Rcombinantly Expressed in E. coli. |
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E.E. Sampson, G.D. Hveman, H.C. Aldrich, T.A. Bobik |
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A. Hasona**, H. Tao |
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R.V. Dumitru, H. Palencia, J.M. Takacs, B. DeMontigny, J. Miner, M. Rasche, S.W. Ragsdale |
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S.A. Wiles, M. Rosentzvaig, M.E. Rasche |
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University of Georgia, Department of Microbiology |
Athens, GA, 2002 |
Julie A. Maupin-Furlow |
Energy-dependent Proteolysis in the Archaea. |
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DARPA Biological Input-Output Systems Program, Kick-off Meeting |
San Diego, CA, June 14-15, 2002 Arlington, VA, May 27-28, 2003 |
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Sentinel Plants Utilizing Bacterial Two-Component Systems. |
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Gordon Conference: Biocatalysis Workshop |
Meredith, NH, July 11-12, 2002 |
Lonnie O. Ingram
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Symposium Speaker |
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Society of Industrial. Microbiology and Biotechnology |
Philadelphia, Aug 2002 |
Symposium Speaker |
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Bioproducts Advisory Group |
Washington, DC., September, 2002 |
Invited Speaker |
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Basic Energy Sciences Advisory Committee-Sponsored Workshop |
Gaithersburg, MD, October 21-24, 2002 |
Basic Research Needs To Assure A Secure Energy Future |
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DOE, DOE, BER Genomes to Life Advisory Group |
Washington, DC. October, 2002 |
Invited Speaker |
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DOE, Basic Energy Science Advisory Group (Future Challenges) |
Washington, DC, November, 2002 |
Invited Speaker |
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Cargill, Inc. |
St. Paul, MN, November 10-11 |
Review Cargill’s Biobased Chemicals Research And Development Program. |
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InterAgency (DARPA, DOE, USDA, NSF, NIH, NIST, ONR) Conference |
Arlington, Virginia, 2001, 2002, January 31, 2003 |
Metabolic Engineering Working Group |
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25th Symposium on Biotechnology for Fuels and Chemicals |
April, 2003 |
Symposium Speaker |
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Annual Meeting of the Amer. Society for Microbiology |
Wash DC. May, 17-19, 2003 |
Symposium Speaker |
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Consortium for Plant Biotechnology Symposium |
Washington DC February 26-27, 2003. |
Maria E. Rodriquez, Alfredo Martinez, Sean W. York, Kheir Zuobi-Hasona, Lonnie O. Ingram, K. T. Shanmugam, and James F. Preston |
Properties of the Hemicellulose Fractions of Lignocellulosic Biomass Affecting Ethanol Production
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James F. Preston, Franz J. St John, John D. Rice, Lonnie O. Ingram, and K. T. Shanmugam |
Utilization of Hemicellulose-derived Glucuronoxylose for Ethanol Production |
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James F. Preston, Franz J. St John, John D. Rice, Lonnie O. Ingram, and K. T. Shanmugam |
Bioconversion of Lignocellulosics to Alternative Fuels and Chemicals |
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25th Symposium on Biotechnology for Fuels and Chemicals |
Washington, DC, May 4-7, 2003 |
K.T. Shanmugam |
Second Generation Biocatalysts for Production of Fuels and Chemicals from Biomass |
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1st Annual Meeting of S1007 – The Science and Engineering for a Biobased Industry and Economy |
Washington, DC May 8-9, 2003 |
K.T. Shanmugam |
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Washington, DC, May 18-22, 2003 |
Lonnie O. Ingram |
Symposium Speaker: Engineering Escherichia coli For The Production Of Higher Value Fermentation Products. |
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Vilas Shukla** |
Production Of Lactic Acid From Sucrose By Metabolic Engineered Escherichia coli. |
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Yilei Qian |
Cloning, Characterization And Functional Expression Of The Klebsiella oxytoca Xyloside Utilization Operon (xynb) In Escherichia coli |
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Stuart Underwood* |
Osmoprotectants Stimulate Growth And Ethanol Production By Ethanologenic Escherichia coli. |
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Franz St. John* |
Xylan Utilizing Paenibacillus sp. Encodes A GH10 Xylanase Containing Triplicate Domains For Cell Surface And Substrate Associations. |
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Bryce Causey* |
Engineering The Metabolism Of Escherichia coli W3110 For Homo-Acetate Production. |
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Steven Kaczowka* |
Recombinant Production Of Zymomonas mobilis Pyruvate Decarboxylase I The Archaeon Haloferax volcanii |
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Shengde Zhou |
Novel Escherichia coli Biocatalysts For The Efficient Production Of L(+) And D(-) Lactic Acids From Hexose And Pentose Sugars In Mineral Salts Medium. |
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Christopher Reuter* |
Proteasome-Activating Nucleotidases From Haloferax volcanii: Subunit Complexity And Activity. |
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Kheir Zoubi-Hasona** |
Growth Dependent Post-Translational Modification Of The Alpha1 Subunit Of Halofrex volcanii 20S. |
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Matt Bechard* |
Overproduction Characterization, And Site-Directed Mutagenesis Of RFAP Synthase From The Methanogen Methanothermobacter thermautrophicus Delta H. |
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Lee Ann Talarico* |
Comparison And Analysis Of Pyruvate Decarboxylase Gene Expression In A Gram-Postive Host. |
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Milind Patel** |
Genetic And Biochemical Characterization Of D-Lactate Dehydrogenase From A Secomd Generation Biocatalyst Producing L-(+) Lactic Acid. |
Faculty members have been actively involved in several academic service activities. They include:
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Faculty Member |
Type of Service |
Insitution/Society |
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Editorial Board |
Journal of Applied and Environmental Microbiology, 1996-2001. |
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Chairman, Fermentation and Biotechnology Division |
American Society for Microbiology (Division O), 2000-2001 |
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Symposium Chairman |
Soc. of Indust. Microbiol. and Biotechnol., Recent Advances in Fermentation Technology IV, October 2001 |
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Fellow |
American Academy of Microbiology, 1998-present |
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Society of Industrial Microbiology and Biotechnology, 2001-present |
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Member |
U.S. National Academy of Sciences, 2001-present |
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Editorial Board |
Biotechnology Letters 1996-present |
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Metabolic Engineering 1998-present |
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Member, Grant Review Panel |
DOE, USDA, Interagency Metabolic Engineering Initiative |
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Grant Proposal Reviewer |
NSF, NIH, DOE, USDA, several state programs, Canada, Australia, Hong Kong, Consortium for Plant Biotechnology Research |
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Conference Organizer |
“Interfacing Microbiology and Biotechnology”, Gainesville on May 17-19, 2001. |
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President, ASM Southeastern Branch |
American Society for Microbiology, 2002 |
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Secretary-Treasurer |
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Local Planners |
Annual Meeting of the Southeastern Branch at Gainesville, Florida. Nov 6-9, 2002 |
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Awards Committee Member |
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Evaluator |
Research and Development Program of Cargill Central Research Division. Nov 2002 |
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Refereed Journal Reviewer |
Science |
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Applied and Environmental Microbiology |
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Journal of Experimental Microbiology |
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Carbohydrate Research |
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Physiological and Molecular Plant Pathology |
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Journal of Bacteriology |
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Member, Faculty Advisory Committee |
Vice-President of IFAS, 2002-2004 |
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Member |
University Senate, 2002-2004 |
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