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Progress Report
The
Florida Center for Renewable
July 2001- June 2003
University of Florida
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CONTACT INFORMATION
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TABLE OF CONTENTS
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Introduction
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Faculty Member/Visiting Scientist |
Place and Date |
Topic |
|
James F. Preston, III |
Department of Microbiology and Cell Science, September 3, 2001. |
“Pasteuria Spp.: Endospore-Forming Bacterial Parasites Of Cladocerans And Phytopathogenic Nematodes”. |
|
Department of Plant Pathology, January 14, 2003. |
“Pasteuria Spp.: Endospore-Forming Bacterial Parasites Of Cladocerans And Phytopathogenic Nematodes As Naturally Occurring Biocontrol Agents.” |
In-house seminars are conducted during the Fall and Spring Semesters. Research progress reports by undergraduate, and graduate students, post-docs, staff members, and research faculty members are presented for discussion and suggestions. Twenty five to thirty people regularly attend this weekly seminar.
Faculty members and their students attended conferences and seminars to present research results and to interact with other researchers from other academic and industrial institutions. The Table in the following pages shows the conference, seminar, or workshop, place and date, and topics presented by attendees.
Table. 1. Conferences, Workshops, and Meetings Attended by
Members of the FCRC.
|
Conference/Seminar/ |
Date and Place |
Attendees/Poster Presenters/Speakers |
Topic |
|
Symposium for the Society of Nematology. |
Salt Lake City, 2001 |
Preston, J.F., D.W. Dickson, J.E. Maruniak, J.A. Brito, L.M. Schmidt, and J.M. Anderson |
Pasteuria Spp.: Systematics-Phylogeny Of These Unusual Bacterial Parasites Of Phytopathogenic Nematodes. |
|
Conference on Interfacing Microbiology and Biotechnology |
University of Florida, Gainesville, FL May 17-19, 2001. |
J.F. Preston, III |
Microbial Strategies For The Depolymerization Of Glucuronoxylan. |
|
Madeline E. Rache |
C1 Metabolism And Methanogens |
||
|
Lonnie O. Ingram and James F. Preston, III |
Metabolic Engineering And Regulation |
||
|
K.T. Shanmugam |
Bioinformatics And Global Gene Expression |
||
|
Julie A. Maupin-Furlow |
Nanocompartments Of Prokaryotic Cells |
||
|
101st American Society for Microbiology General Meeting |
Orlando, FL. May 20-24, 2001. |
Lonnie O. Ingram
|
Symposium Speaker: Genetic Engineering For Production Of Industrial Chemicals. |
|
Rebecca M. Harbucker* |
Isolation Of A Thermotolerant Acid Resistant Host For The adh And pdc Genes Of Zymomonas mobilis To Enable Cost Effective Production Of Ethanol For Biomass. |
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|
ME Rodriguez* |
Properties Of The Hemicellulose Fractions Of Lignocellulosic Biomass Affecting Bacterial Ethanol Production. |
||
|
Tony Gutierrez* |
Biotransformation Of Furfural By Ethanologenic Bacteria And Its Effect On Ethanol Production. |
||
|
Lee Ann Talarico* |
Cloning And Characterization Of Pyruvate Decarboxylase From SarcinavVentriculi. |
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|
FrankG. Healy* |
Development Of Ethanologenic Escherichia coli For The Simultaneous Utilization Of Glucose And Xylose. |
||
|
Stuart A. Underwood* |
Additional Osmoprotectants Are Required For The Efficient Fermentation Of 10% Xylose To Ethanol In Nutrient-Limiting Media |
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|
Ramon Gonzalez* |
Identification Of Promoters For Programmed Expression Of Recombinant Products Im Escherichia Coli By Transcriptone Analysis. |
||
|
Steven J. Kaczowka* |
Structure And Function Of Proteasomes From A Halophilic Archaeon Halofreax volcanii. |
||
|
H.L. Wilson* |
Purification And Characterization Of The 20S Proteasome And PAN From Methanococcus maripaludis. |
||
|
Mark S. Ou* |
Influence Of The Alpha Subunit On The Proteolytic Activity Of 20S Proteosomes |
||
|
Kheir Zuobi-Hasona** |
Oligosaccharides Containing Glucuronoxylose As Substrates For Selecting Bacteria For Depolymerization Of Hemicellulose. |
||
|
Shengde Zhou |
Fermentation Of Cellulooligosaccharides And Amorphous Cellulose To Ethanol By Cellulolytic Detivatives Of Klebsiella oxytoca. |
||
|
A.M. Paredes, F. Soyer, H.C. Aldrich, S. Lutke, H Tsuruta, W.Chiu, JM Shively |
Three-dimensional structure of Thiobacilllus neapolitanus Carboxysomes as Determined by Cryo-electon Microscopy. |
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|
E.M. Sampson, G.D. Haveman, H.C. Aldrich, T.A. Bobik |
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|
Gordon Research Conference on Archaea: Ecology, Metabolism, and Molecular Biology |
2001 Proctor Academy, NH |
Steven Kaczowka* |
Localization Of The Alpha-1 And Alpha-2 Subunits Of 20S Proteasomes From A Halophilic Archaeon, Haloferax volcanii’ |
|
2nd Gordon Conference on Molybdenum and Tungsten Enzymes |
Queen’s College, Oxford, England July 1-6, 2001 |
K.T. Shanmugam |
Molybdenum Metabolism. |
|
University of Florida, Dept. of Microbiology and Cell Science |
Gainesville, FL, 2001 |
Julie A. Maupin-Furlow |
Energy-dependent Proteolysis in the Archaea. |
|
Gordon Research Conference, Archaea: Ecology, metabolism, and Microbiology |
Andover, NH, 2002 |
Julie A. Maupin-Furlow |
Archael Proteosomes and Proteosome-Activating Nucleotidases. |
|
Brookhaven National Laboratories |
Upton, NY, 2001 |
Julie A. Maupin-Furlow |
Proteosomes: Molecular Machines Integral to the Genomes to Life Initiative. |
|
223rd National Meeting of the American Chemical Society. |
Orlando, FL April 7-11, 2002. |
Preston, J.F., and J.C. Hurlbert |
Microbial Strategies For The Depolymerization Of Methylglucuronoxylan.- Payen Award Symposium Honoring Liisa Viikari: Application Of Enzymes To Lignocellulosics. |
|
23rd Symposium on Biotechnology for Fuels and Chemicals |
Breckenridge, Colorado May 4-7, 2003 |
Stuart Underwood* |
Optimizing Carbon Partitioning between Biosynthesis and Ethanol Production in Ethanologenic Escherichia coli KO11 |
|
James F. Preston, Franz J. St. John and John D. Rice |
Selection of Endoxylanases for Depolymerization of Glucuronoxylan from Hemicellulose |
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|
Annual Meeting of the American Society for Microbiology Southeastern Branch |
Gainesville, Florida, on November 7-9, 2002 |
Lonnie O. Ingram |
Engineering E. coli For Renewable Chemicals And Fuels. |
|
K.T. Shanmugam |
Whole Genome Expression Analysis Of E. coli Growing Under Aerobic And Anaerobic Condition. |
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|
Bryce Causey* |
Microbial Platform For The Production Of Oxidized Compounds (Acetate And Pyruvate) From Escherichia coli W3110 ATCC. |
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|
Lee Ann Talarico* |
Characterization Of Pyruvate Decarboxylase From Sarcina ventriculi. |
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|
Franz St. John* |
Xylan Utilization By Paenibacillus Sp Secresting a GH10 Xylanase With Significant Homology To GH10 Of Clostridium josui. |
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|
Stuart Underwood* |
Balancing Carbon Partitioning Between Biosynthesis And Product Formation |
||
|
Shengde Zhou |
Metabolic Engineering Escherichia coli W3110 Fro Production Of Optically Pure D(-) And L(+)-Lactic Acid From Mineral Salts Medium. |
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|
Christopher Reuter* |
Biochemical Characterization And Subunit Arrangement Of PAN Proteins From Halophilic Archeon Haloferax volcanii. |
||
|
Steven J. Kaczowka* |
Subunit Arrangement Of 20S Proteosomes From Haloferax volanii. |
||
|
Phoung-Uyen Le*** |
Thermostability Of Sarcina ventriculi Pyruvate Decarboxylase At Ph 5.5 |
||
|
Brea Duval*** |
Generation Of Coupled Microtiter Plat Assay For Sarcina ventriculi Pyruvate Decarboxylase. |
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|
Cheryl Boice* |
Characterization Of Bacteriphages For Use In Adsorption And Transport Studies. |
||
|
Johnny Davis* |
Fate Of Bacterial Indicators And Pathogens In Anaerobically Digested Dairy Wastewater. |
||
|
Yilei Qian |
Isolation Of The Xylosidase Gene (Xynb) Operon From Klebsiella oxytoca. |
||
|
Liesbeth Schmidt** |
Detection Of Pasteuria penetrans P20 Infected Meloidogyne arenaria Race 1 By Polymerase Chain Reaction (PCR). |
||
|
Vilas Shukla** |
Engineering Escherichia coli For Lactic Acid Production From Sucrose. |
||
|
Brent Wood* |
Reprioritization Of Sugar Utilization Profiles By Overexpression Of A CRP* In Escherichia coli KO11 |
||
|
Annual Meeting of the American Society for Microbiology |
Salt Lake City, Utah, 2002 |
Julie A. Maupin-Furlow |
Compartmentalization of Proteolysis Mediated by the Archaeal Proteosome. |
|
G.C. Cannon, S. Heinhorst, H.C. Aldrich, E. Williams, J. Shively |
Characterization of Halothiobacillus neapolotanus Carboxysomes Rcombinantly Expressed in E. coli. |
||
|
E.E. Sampson, G.D. Hveman, H.C. Aldrich, T.A. Bobik |
|||
|
A. Hasona**, H. Tao |
|||
|
R.V. Dumitru, H. Palencia, J.M. Takacs, B. DeMontigny, J. Miner, M. Rasche, S.W. Ragsdale |
|||
|
S.A. Wiles, M. Rosentzvaig, M.E. Rasche |
|||
|
University of Georgia, Department of Microbiology |
Athens, GA, 2002 |
Julie A. Maupin-Furlow |
Energy-dependent Proteolysis in the Archaea. |
|
DARPA Biological Input-Output Systems Program, Kick-off Meeting |
San Diego, CA, June 14-15, 2002 Arlington, VA, May 27-28, 2003 |
|
Sentinel Plants Utilizing Bacterial Two-Component Systems. |
|
Gordon Conference: Biocatalysis Workshop |
Meredith, NH, July 11-12, 2002 |
Lonnie O. Ingram
|
Symposium Speaker |
|
Society of Industrial. Microbiology and Biotechnology |
Philadelphia, Aug 2002 |
Symposium Speaker |
|
|
Bioproducts Advisory Group |
Washington, DC., September, 2002 |
Invited Speaker |
|
|
Basic Energy Sciences Advisory Committee-Sponsored Workshop |
Gaithersburg, MD, October 21-24, 2002 |
Basic Research Needs To Assure A Secure Energy Future |
|
|
DOE, DOE, BER Genomes to Life Advisory Group |
Washington, DC. October, 2002 |
Invited Speaker |
|
|
DOE, Basic Energy Science Advisory Group (Future Challenges) |
Washington, DC, November, 2002 |
Invited Speaker |
|
|
Cargill, Inc. |
St. Paul, MN, November 10-11 |
Review Cargill’s Biobased Chemicals Research And Development Program. |
|
|
InterAgency (DARPA, DOE, USDA, NSF, NIH, NIST, ONR) Conference |
Arlington, Virginia, 2001, 2002, January 31, 2003 |
Metabolic Engineering Working Group |
|
|
25th Symposium on Biotechnology for Fuels and Chemicals |
April, 2003 |
Symposium Speaker |
|
|
Annual Meeting of the Amer. Society for Microbiology |
Wash DC. May, 17-19, 2003 |
Symposium Speaker |
|
|
Consortium for Plant Biotechnology Symposium |
Washington DC February 26-27, 2003. |
Maria E. Rodriquez, Alfredo Martinez, Sean W. York, Kheir Zuobi-Hasona, Lonnie O. Ingram, K. T. Shanmugam, and James F. Preston |
Properties of the Hemicellulose Fractions of Lignocellulosic Biomass Affecting Ethanol Production
|
|
James F. Preston, Franz J. St John, John D. Rice, Lonnie O. Ingram, and K. T. Shanmugam |
Utilization of Hemicellulose-derived Glucuronoxylose for Ethanol Production |
||
|
James F. Preston, Franz J. St John, John D. Rice, Lonnie O. Ingram, and K. T. Shanmugam |
Bioconversion of Lignocellulosics to Alternative Fuels and Chemicals |
||
|
25th Symposium on Biotechnology for Fuels and Chemicals |
Washington, DC, May 4-7, 2003 |
K.T. Shanmugam |
Second Generation Biocatalysts for Production of Fuels and Chemicals from Biomass |
|
1st Annual Meeting of S1007 – The Science and Engineering for a Biobased Industry and Economy |
Washington, DC May 8-9, 2003 |
K.T. Shanmugam |
|
|
Washington, DC, May 18-22, 2003 |
Lonnie O. Ingram |
Symposium Speaker: Engineering Escherichia coli For The Production Of Higher Value Fermentation Products. |
|
|
Vilas Shukla** |
Production Of Lactic Acid From Sucrose By Metabolic Engineered Escherichia coli. |
||
|
Yilei Qian |
Cloning, Characterization And Functional Expression Of The Klebsiella oxytoca Xyloside Utilization Operon (xynb) In Escherichia coli |
||
|
Stuart Underwood* |
Osmoprotectants Stimulate Growth And Ethanol Production By Ethanologenic Escherichia coli. |
||
|
Franz St. John* |
Xylan Utilizing Paenibacillus sp. Encodes A GH10 Xylanase Containing Triplicate Domains For Cell Surface And Substrate Associations. |
||
|
Bryce Causey* |
Engineering The Metabolism Of Escherichia coli W3110 For Homo-Acetate Production. |
||
|
Steven Kaczowka* |
Recombinant Production Of Zymomonas mobilis Pyruvate Decarboxylase I The Archaeon Haloferax volcanii |
||
|
Shengde Zhou |
Novel Escherichia coli Biocatalysts For The Efficient Production Of L(+) And D(-) Lactic Acids From Hexose And Pentose Sugars In Mineral Salts Medium. |
||
|
Christopher Reuter* |
Proteasome-Activating Nucleotidases From Haloferax volcanii: Subunit Complexity And Activity. |
||
|
Kheir Zoubi-Hasona** |
Growth Dependent Post-Translational Modification Of The Alpha1 Subunit Of Halofrex volcanii 20S. |
||
|
Matt Bechard* |
Overproduction Characterization, And Site-Directed Mutagenesis Of RFAP Synthase From The Methanogen Methanothermobacter thermautrophicus Delta H. |
||
|
Lee Ann Talarico* |
Comparison And Analysis Of Pyruvate Decarboxylase Gene Expression In A Gram-Postive Host. |
||
|
Milind Patel** |
Genetic And Biochemical Characterization Of D-Lactate Dehydrogenase From A Secomd Generation Biocatalyst Producing L-(+) Lactic Acid. |
Faculty members have been actively involved in several academic service activities. They include:
|
Faculty Member |
Type of Service |
Insitution/Society |
|
Editorial Board |
Journal of Applied and Environmental Microbiology, 1996-2001. |
|
|
Chairman, Fermentation and Biotechnology Division |
American Society for Microbiology (Division O), 2000-2001 |
|
|
Symposium Chairman |
Soc. of Indust. Microbiol. and Biotechnol., Recent Advances in Fermentation Technology IV, October 2001 |
|
|
Fellow |
American Academy of Microbiology, 1998-present |
|
|
Society of Industrial Microbiology and Biotechnology, 2001-present |
||
|
Member |
U.S. National Academy of Sciences, 2001-present |
|
|
Editorial Board |
Biotechnology Letters 1996-present |
|
|
Metabolic Engineering 1998-present |
||
|
Member, Grant Review Panel |
DOE, USDA, Interagency Metabolic Engineering Initiative |
|
|
Grant Proposal Reviewer |
NSF, NIH, DOE, USDA, several state programs, Canada, Australia, Hong Kong, Consortium for Plant Biotechnology Research |
|
|
Conference Organizer |
“Interfacing Microbiology and Biotechnology”, Gainesville on May 17-19, 2001. |
|
|
President, ASM Southeastern Branch |
American Society for Microbiology, 2002 |
|
|
Secretary-Treasurer |
||
|
Local Planners |
Annual Meeting of the Southeastern Branch at Gainesville, Florida. Nov 6-9, 2002 |
|
|
Awards Committee Member |
||
|
Evaluator |
Research and Development Program of Cargill Central Research Division. Nov 2002 |
|
|
Refereed Journal Reviewer |
Science |
|
|
Applied and Environmental Microbiology |
||
|
Journal of Experimental Microbiology |
||
|
Carbohydrate Research |
||
|
Physiological and Molecular Plant Pathology |
||
|
Journal of Bacteriology |
||
|
Member, Faculty Advisory Committee |
Vice-President of IFAS, 2002-2004 |
|
|
Member |
University Senate, 2002-2004 |
|
|
Panel Member, Grant Proposal Review Board |
National Science Foundation, 2002 |
|
|
Grant Proposal Reviewer |
National Science Foundation, 1999-2003 |
Outstanding graduate students received travel grants to present their research results at regional and national meetings, and awards for best research presentations. Sponsors include: American Society for Microbiology, Institute of Agricultural and Life Sciences, Gordon Research Conference on Archea, UF Student Government, and Kikoman Corporation.
|
Student |
Award |
Sponsor/Purpose |
|
Steven Kaczowka |
ASM Sustaining Member Student Travel Award |
101th American Society for Microbiology General Meeting, Orlando, FL. May 20-24, 2001 |
|
Gordon Research Conference Travel Award |
Gordon Research Conference on Archaea: Ecology, Metabolism, and Molecular Biology 2001 Proctor Academy, NH |
|
|
Brea Duval |
President’s Award for Best Paper Presentation by an Undergraduate Student. |
American Society for Microbiology
General Meeting, Southeast Branch, Gainesville, Fl,
|
|
Lee Ann Talarico |
President’s Award for Best Paper Presentation by a Graduate Student. |
|
|
The Kikoman Corporation Student Travel Award |
American Society for Microbiology Annual Meeting, Washington, DC, May 18-22, 2003 |
|
|
Stuart Underwood |
ASM Division O Student Travel Award |
|
|
Bryce Causey |
ASM Corporate Accounts Program, Division O Student Travel Grant |
|
|
Bryce Causey, |
IFAS $200 Travel Grant |
|
|
Stuart Underwood |
Student Government Travel Grant |
Dr. Jimmy G. Cheek, Dean of the College of Agricultural and Life Sciences, IFAS, granted four matching graduate assistantships in Fall 2002 and five additional slots this Fall 2003:
|
Graduate Assistant |
Date Granted |
Degree |
Mentor |
|
Maegorzata Gil |
2002 |
MS |
Dr. Julie A. Maupin-Furlow |
|
Jeremy Purvis |
2002 |
MS |
Dr. Lonnie O. Ingram |
|
Franz St. John |
MS |
Dr. James F. Preston, III |
|
|
Kim Youngyun |
2002 |
MS |
Dr. K.T. Shanmugam |
|
Phi Min Do |
2003 |
MS |
Dr. K. T. Shanmugam* |
|
Phillip Kirkland |
2003 |
MS |
Dr. Julie A. Maupin-Furlow* |
|
Shannon McQuaig |
2003 |
MS |
Dr. Samuel R. Farrah* |
|
Jonathan Moore |
2003 |
MS |
Dr. James F. Preston, III* |
|
Amy Wright |
2003 |
MS |
Dr. Madeline Rasche* |
* provisional mentors; students go on rotation to different labs before choosing their home lab
The Center currently supports 7 other graduate students, 10 post-docs, and 4 research faculty members:
|
Name |
Degree/Position |
Mentor/Lab |
|
MS |
Dr. Madeline Rasche |
|
|
Bryce Causey |
PhD |
Dr. Lonnie O. Ingram |
|
Shai Lawit |
MS |
Dr. William B. Gurley |
|
Steven Kaczowka |
MS |
Dr. Julie A. Maupin-Furlow |
|
Christophen Reuter |
MS |
Dr. Julie A. Maupin-Furlow |
|
Lee Ann Talarico |
PhD |
Dr. Julie A. Maupin-Furlow |
|
Brent Wood |
PhD |
Dr. Lonnie O. Ingram |
|
Patrick Cirino |
Visiting Scientist |
Dr. Lonnie O. Ingram |
|
Yilei Qian |
Research Faculty |
Dr. Lonnie O. Ingram |
|
Shengde Zhou |
Research Faculty |
Dr. Lonnie O. Ingram |
|
Stuart Underwood |
Post-Doc |
Dr. Lonnie O. Ingram |
|
Eun Min Cho |
Post-Doc |
Dr. Nemat Keyhani |
|
Eva Czarnecka-Verner |
Research Faculty |
Dr. William B. Gurley |
|
Haeng Seorg Lee |
Post-Doc |
Dr. William B. Gurley |
|
Kevin O’Grady |
Post-Doc |
Dr. William B. Gurley |
|
Tarek Salem |
Post-Doc |
Dr. William B. Gurley |
|
Ivanka Karadzic |
Post-Doc |
Dr. Julie Maupin-Furlow |
|
Guang Nong |
Post-Doc |
Dr. James F. Preston, III |
|
Liesbeth Schmidt |
Post-Doc |
Dr. James F. Preston, III |
|
Virginia Chow |
Post-Doc |
Dr. James F. Preston, III |
|
Tao Han |
Research Faculty |
Dr. K.T. Shanmugam |
|
Milind Patel |
Post-Doc |
Dr. K.T. Shanmugam |
Application Number |
Date Approved |
Country |
Authors |
Title |
|
ZL92101877.0 |
01/27/01 |
China |
Lonnie O. Ingram, |
Ethanol Production by Recombinant Hosts
|
|
6,569,632 |
|
Preston, James F., D.W. Dickson, John D. Rice, J.H. Charnecki, J.M. Brito, Liesbeth M. Schmidt |
Monoclonal Antibody Probe for Detection of Adhesins Associated with Mature Endospores of Pasteuria Spp |
|
Application Number |
Date Submitted |
Authors |
Title |
|
60/424,372 |
11/06/02 |
Lonnie O. Ingram, Thomas B. Causey, Shengde Zhou, K. T. Shanmugam |
Materials and Methods for the Efficient Production of Acetate and other Products |
|
10/003,741 |
11/02/02 |
Lonnie O. Ingram, Jacob L. Snoep, Nico Arfman |
Recombinant Glucose Uptake System |
|
60/418,069 |
10/11/02 |
Lonnie O. Ingram, Shengde Zhou, Thomas B. Causey, K . T. Shanmugam |
Mutation to Increase Production of Fermentation Products |
|
60/378,143 |
05/13/02 |
Adnan Hasona, K. T. Shanmugam, Lonnie O. Ingram |
Novel Pseudomonas Putida EstZ Esterase Gene and Uses Thereof |
|
10/136,960 |
04/30/02 |
Julie A. Maupin-Furlow, Lee Ann Talarico, Krishnan Chandra Raj, Lonnie O. Ingram |
Cloning and Sequencing of Pyruvate Decaroxylase (PDC) Genes from Bacteria and Uses Thereof |
|
08/834,900 |
04/16/02 |
Lonnie O. Ingram, Lorraine Yomano,Sean York |
Development of High-Ethanol Resistant Escherichia coli |
|
10/027,250 |
12/21/01 |
Brent Wood, Lonnie O. Ingram |
Improvement of Ethanol Production from Lignocellulose |
|
10/024,456 |
12/17/01 |
Lonnie O. Ingram, Sean W. York |
Ethanol Production Using a Soy Hydrolysate-based Medium or a Yeast Autolysate-based Medium |
|
10/001,218 |
11/30/01 |
Lonnie O. Ingram, Maria Barbosa-Alleyne |
Ethanol Production in Gram-Positive Microbes |
|
09/885,297 |
06/19/01 |
Shengde Zhou, |
Methods and Compositions for Simultaneous Saccharification and Fermentation |
|
09/885,294 |
06/19/01 |
Lonnie O. Ingram, |
Methods for Improving Cell Growth and Alcohol Production During Fermentation. |
|
60/288,699 |
05/04/01 |
Lonnie O. Ingram, |
Pyruvate Decarboxylase: A Key Enzyme for the Oxidative Metabolism of Lactic Acid by Acetobacter pasteurianus |
|
60/288,698 |
05/04/01 |
Lonnie O. Ingram, |
Acetobacter Patstuerianus Pyruvate Decarboxylase: Biochemical, Genetic, and Physiological Properties |
|
60/288,671 |
05/04/01 |
Lonnie O. Ingram, |
Cloning, Expression, and Characterization of Pyruvate Decarboxylase fro the Acod Tolerant, Anaerobic Gram-Positive Bacteriun Sarcinia ventriculi9 Goodsir |
|
60/288,638 |
05/04/01 |
Lee Ann Talarico, Lonnie O. Ingram, |
High-level Production of Active Sarcina ventriculi Pyruvate Decarboxylase in Recombinant Bacillus megaterium |
|
60/288,622 |
05/04/01 |
Chandra Raj Krishan, Lonnie O.
Ingram, |
Biochemical and Biophysical characterization of Pyruvate Decarboxylase from the Acetic Acid Bacterium Acetobacter Pastuerianus |
International patent applications (17):
ApplicationNumber |
Date Submitted |
Country |
Authors |
Title |
|
P 02 01 01630 |
05/03/02 |
Argentina |
Julie A. Maupin-Furlow, Lee Ann Talarico, Krishnan Chandra Raj, Lonnie O. Ingram |
Cloning and Sequencing of Pyruvate Decarboxylase(PDC) Genes from Bacteria and Uses Thereof |
|
P 01 01 03040
0946576.4-4-2405-US0119690 |
06/26/01 |
Argentina Thailand PCT |
Shengde Zhou,
|
Methods and Compositions for Simultaneous Saccharification and Fermentation |
|
066459 |
06/22/01 |
Thailand |
Lonnie O. Ingram, |
Methods for Improving Cell Growth and Alcohol Production During Fermentation. |
|
02106753.2 |
09/13/02 |
Hongkong |
Lonnie O. Ingram, |
Recombinant Hosts suitable for simultaneous Saccharification and Fermentation |
Rasche, M. E., Wyles,
S. A., and Rosenzvaig, M. 2004. Characterization of two methanopterin
biosynthesis mutants of Methylobacterium extorquens AM1 using a
tetrahydromethanopterin bioassay. J. Bacteriol. (Accepted with revision)
Caccamo, M. A., Malone, C. S., and Rasche, M. E. 2004. Biochemical
characterization of a dihydromethanopterin reductase involved in
tetrahydromethanopterin biosynthesis in Methylobacterium extorquens
AM1. J. Bacteriol. (Accepted with revision)
Kima, P.E, Rasche, M.E. 2004. Sex determination using the polymerase chain
reaction. Biochemistry and Molecular Biology Education (Accepted).
Rasche, M. E. 2003. Outcomes of a research-driven laboratory and literature
course designed to enhance undergraduate contributions to original
research. Biochemistry and Molecular Biology Education.
Bobik, T.A., Rasche, M.E. 2003. Purification and partial characterization
of the Pyrococcus horikoshii methylmalonyl-CoA epimerase. Applied
Microbiology and Biotechnology.
Dumitru, R., Palencia., H., Schroeder, S. D., DeMontigny, B., Takacs, J.,
Rasche, M. E., Miner, J. L., and Ragsdale, S. W. 2003. Targeting
methanopterin biosynthesis to inhibit methanogenesis. Appl. Environ.
Microbiol.
Kaczowka, S.J. and J.A. Maupin-Furlow. 20003. Archael Ptroteasomes: from
structure to function [update 2003]. Front. Biosci.
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Schmidt, L. M., J. F. Preston, D. W.
Dickson, J. D. Rice, T. W. Hewlett. 2003. Environmental quantification
of Pasteuriapenetrans endospores using in situ antigen extraction
and immunodetection with a monoclonal antibody. FEMS Microbiol. Ecol.
44:17-26.Z |
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Maupin-Furlow, J.A., S.J. Kaczowka, C.J.
Reuter, K. Zuobi-Hasona, and M.A. Gil. 2003. Archael proteasomes:
potential in metabolic engineering. Metab. Engin. 5:151-163. |
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Kaczowka, S.J. and J.A. Maupin-furlow.
2003. Subunit topology of the multiple 20S proteasomes from Haloferax
volcanii. J. Bacteriology 185:165-174. |
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Preston, J. F., D.
W. Dickson, J. E. Maruniak, G. Nong, J. A. Brito, L. M. Schmidt, and R.
M. Giblin-Davis. 2003. Pasteuria spp. : Systematics and
phylogeny of these bacterial parasites of phytopathogenic nematodes.
Journal of Nematology 35: 198-207 |
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Brito, J. A, J. F.
Preston, D. W.Dickson, R. M. Giblin-Davis, D. S. Williams, H. C.
Aldrich, and J. D. Rice. 2003. Temporal Production and
Immunolocalization of an Epitope During Pasteuria penetrans
sporogenesis. Journal of Nematology 35(3): |
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Bobik, T. A.,
and Rasche, M. E. 2003. HPLC assay for methylmalonyl-CoA epimerase.
Anal. Bioanal. Chem.
375:344-349. |
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Uz, I, Rasche, M.E.,
Townsend, T., Ogram, A. V., and Lindner, A. S.
2003. Characterization of methanogenic and methanotrophic assemblages
in landfill samples. Proceedings of the Royal Society of London B
(Biology Letters Suppl.) 270:S202-S205. |
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Bechard ME, Chhatwal S, Garcia RE,
Rasche ME. 2003. Application of a colorimetric assay to identify
putative RFAP synthase genes expressed with activity in Escherichia
coli. Biological Procedures Online 5:69-77. |
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Eckert,
Richard B., Henry C. Aldrich, Chris A. Edwards, Bruce A. Cookingham.
2003. Microscopic differentiation of internal corrosion initiation
mechanisms in natural gas pipeline systems. Corrosion, San Diego, CA |
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Bogan,
Bill W., Wendy R. Sullivan, Kevin J. Kayser, KD Derr, Henry C. Aldrich
and J. Robert Paterek.
2003. Alkanindiges illinoisensis gen. nov., sp. nov., an obligately
hydrocarbonoclastic, aerobic squalane-degrading bacterium isolated from
oilfield soils. Int J Syst Evol Microbiol 53: 1389-1395. |
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Gonzalez, R.,
H. Tao, J. E. Purvis, S. W. York, K. T. Shanmugam and L. O. Ingram.2003.
Gene array-based identification of beneficial changes that contribute to
increased ethanol tolerance in ethanologenic Escherichia coli
LY01. Biotechnol. Prog. 19:612-623.
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Zhou, Shengde,
K.T. Shanmugam and L.O. Ingram. 2003. Functional replacement of
Escherichia coli D(-)-lactate dehydrogenase (ldhA) with
L(+) -lactate dehydrogenase (ldhL) from Pediococcus
acidilactici. Appl. Environ. Microbiol. 60(4): 2237-2244.
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Causey, T. B., S. Zhou, K.
T. Shanmugam, and L. O. Ingram. 2003. Engineering the metabolism of
Escherichia coli W3110 for the conversion of sugar to redox-neutral
and oxidized products: homo-acetate production. Proc. Natl. Acad. Sci.
USA. 100:825-832. |
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| Zhou, S., T.B. Causey, A. Hasona, K.T. Shanmugam, and L.O. Ingram. 2003. Production of optically pure D-lactic acid in mineral salts medium by metabolically engineered Escherichia coli W3110. Appl. Environ. Microbiol. 69(1):399-407. | |||
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Giblin-Davis, R.M.,
D. S. Williams, S. Bekal, D. W. Dickson, J. A. Brito, J. O. Becker and
J. F. Preston. 2003 'Candidatus Pasteuria usgae' sp. nov., an obligate endoparasite
of the phytoparasitic nematode Belonolaimus longicaudatus.
Int J Syst Evol Microbiol 53: 197-200. |
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Scott J.W., ME Rasche. 2002.
Purification, overproduction, and partial characterization of beta-RFA-P
synthase, a key enzyme in the pathway of methanopterin biosynthesis. J.
Bacteriol. 184:4442-4448. |
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Hurlbert, J.C. and J.F. Preston. 2002. Differences
in the solution structures of the parallel Beta-helical pectate lyases
as determined by limited proteolysis. Biochimica et Biophysica Acta.
1599:9-20.
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Underwood,
S.A.,S. Zhou, T. B. Causey, L.P. Yomano, K.T. Shanmugam, and L.O.
Ingram. 2002. Genetic changes to optimize carbon partitioning between
ethanol and biosynthesis ethanologenic Escherichia coli. Appl.
Environ. Microbiol. 68(12): 6263-6272.
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Raj, K.C., L.A. Talarico, L.O. Ingram and J.A. Maupin-Furlow. 2002. Cloning and characterization of the Zymobacter palmae pyruvate decarboxylase (pdc): Comparison of bacterial homologoues. Appl. Environ. Microbiol. 68(6): 2869-2876. |
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Gutierrez, T.,
M.L. Buszko, L.O. Ingram, and J.F. Preston. 2002. Reduction of furfural
to furfuryl alcohol by ethanologenic strains of bacteria and its effect
on ethanol production from xylose. Appl. Biochem. Biotechnol. 98-100:
327-340.
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Hasona, A., S.W. York, L.P. Yomano, L.O. Ingram
and K.T. Shanmugam. 2002. Decreasing the level of ethyl acetate in
ethanologenic fermentation broths of Escherichia coli KO11 by
expression of Pseudomonas putida estZ esterase. Appl. Environ.
Microbiol. 68(6): 2651-2659.
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Underwood, S.A.,
M.L. Buszko, K.T. Shanmugam, and L.O. Ingram. 2002. Flux through citrate
synthase limits the growth of ethanologenic Escherichia coli KO11
during xylose fermentation. Appl. Environ. Microbiol. 68: 1071-1081.
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Gonzalez, R. Han
Tao, K.T. Shanmugam, S. W. York, and L. O. Ingram. 2002. Global gene
expression differences associated with changes in glycolytic flux and
growth rate in Escherichia coli during the fermentation of
glucose and xylose. Biotechnol. Prog. 18: 6-20.
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Bobik TA,
Rasche M. E. 2001. Identification of the human methylmalonyl-CoA
racemase gene based on the
analysis of prokaryotic gene arrangements. Implications for decoding the
human genome. J. Biol. Chem. 276:37194-37198. |
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Preston, J.F., D.W.
Dickson, J.E. Maruniak, J.A. Brito, L.M. Schmidt, and J.M. Anderson.
2001. Pasteuria spp.: Systematics-phylogeny of these unusual
bacterial parasites of phytopathogenic nematodes. Phytopathology.
91:S173. |
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Raj, K. C., L.O.
Ingram and J. A. Maupin-Furlow. 2001. Pyruvate decarboxylase: a key
enzyme for the oxidative metabolism of lactic acid by Acetobacter
pasteurianus. Arch. Microbiol. 176: 443-451. |
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Hurlbert, J.C., and J.F. Preston. 2001. Fuctional
characterization of a novel xylanase from corn strains of Erwinia
chrysanthemi. J. Bacteriol.183: 2093-2100.
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Hasona, A., Self,
W.T. and K. T. Shanmugam. 2001. Transcriptional regulation of the moe
(molybdate transport) operon of Escherichia coli. Arch. Microbiol.
175:178-188. |
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Self, W. T., A. M.
Grunden, A. Hasona and K. T. Shanmugam. 2001. Molybdate transport. Res.
Microbiol. 152:311-321. |
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Self, W. T., A. Hasona and K. T.
Shanmugam. 2001. N-terminal truncations in the FhlA protein result in
formate- and MoeA-independent expression of the hyc operon (formate
hydrogenlyase) of Escherichia coli. Microbiology. 147:3093-3104.
Talarico, L. A., L.O. Ingram and J.A. Maupin-Furlow. 2001. Production of Gram-positive Sarcina ventriculi pyruvate decarboxylase in Escherichia coli. Microbiology-SGM 147:2425-2433. |
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Zhou, S. and L. O.
Ingram. 2001. Simultaneous saccharification and fermentation of
amorphous cellulose to ethanol by recombinant Klebsiella oxytoca
SZ21 without supplemental cellulase. Biotechnol. Lett. 23: 1455-1462.
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Han, T., R.
Gonzales, A. Martinez, M. Rodriguez, L.O. Ingram, J.F. Preston, and K.T.
Shanmugam. 2001. Engineering a homo-ethanol pathway in Eschericia coli:
increased glycolytic flux and levels of expression of glycolytic genes
during xylose fermentation. J. Bacteriol. 183: (10): 2979-2988. |
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Martinez, A., M.E.
Rodriguez, M.L. Wells, S.W. York, J.F. Preston, and L.O. Ingram. 2001.
Detoxification of dilute acid hydrolysates of lignocellulose with lime.
Biotechnol. Prog. 17: 287-293. |
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Zhou, S., F.C.
Davis, and L.O. Ingram. 2001. Gene integration and expression,a nd
extracellular secretion of Erwinia chrysanthemi endoglucanase
CelY (celY) and CelZ (celZ) in ethanologenic Klebsiella
oxytoca P2. Appl. Environ. Microbiol. 67 (1): 6-14.
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Other Publications:
Website: The Florida
Center for Renewable Chemicals and Fuels, 2002.
http://fcrc.ifas.ufl.edu |
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The Center would like to acknowledge all its supporters and sponsors:
UF/IFAS:
Dr. Winfred M. Phillips. Vice President for Research
Dr. Michael V. Martin, Vice President for Agriculture and Natural Resources
Dr. Joseph C. Joyce, Executive Associate
Vice President for Agriculture and
Natural Resources and Budget Director
Dr. Richard L. Jones, Dean for Research and FAES
Dr. William F. Bown, Associate Dean for Research and FAES
Dr. Jimmy Cheek, Dean for Academic Programs and the
College of Agriculture and Life Sciences
Department of Microbiology and Cell Science
Grant Agencies:
Biotechnology and Research Development Corporation (BRDC)
Consortium for Plant Biotechnology, Inc. (CPBR)
Defense Advanced Research Project Agency (DARPA/USDD)
National Institutes of Health
National Science Foundation
Orange County, Florida
US Department of Agriculture
US Department of Energy
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