CONTACT INFORMATION
Dr. Lonnie
O. Ingram
Director
Bldg. 981
Museum Road, PO Box 110700
Gainesville,
FL 32611-0700
Tel: (352)
392-8176; Fax: (352) 846-0969
ingram@ufl.edu
Dr.
Keelnatham T. Shanmugam
Associate
Director
Bldg. 981
Museum Road, PO Box 110700
Gainesville,
FL 32611-0700
Tel: (352)
392-2490; Fax: (352) 392-5922
shan@ufl.edu
Dr.
Sheilachu P. Gomez
Assistant
Director
Bldg. 981
Museum Road, PO Box 110700
Gainesville,
FL 32611-0700
Tel/Fax:
(352) 392-00237
spgomez@ufl.edu
Florida
Center for Renewable Chemicals and Fuels (FCRC)
University
of Florida, Institute of Food and Agricultural Sciences
Department
of Microbiology and Cell Science
Bldg. 981
Museum Road, PO Box 110700
Gainesville,
FL 32611-0700
http://fcrc.ifas.ufl.edu
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|
he Florida Center for
Renewable Chemicals and Fuels (FCRC) is in its third year of operation.
FCRC continues to seek and implement novel solutions to the conversion of
biomass to chemicals and fuels using metabolically engineered bacteria.
This is FCRC’s contribution to reduce the nation’s dependence on imported
petroleum.
FCRC coordinates
bioproducts research by providing a liaison with granting agencies,
assists in the development of competitive grants, and facilitates graduate
education through graduate assistantships, and seminar series featuring
student and faculty researchers.
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Conferences, Seminars, and
Workshops
In-house seminars were
conducted during the Fall and Spring Semesters. Research progress reports
by undergraduate, and graduate students, post-docs, staff members, and
research faculty members were presented for discussion and comments.
Twenty five to thirty people regularly attended this weekly seminar.
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Faculty members, post-docs, and their students
attended various national and international conferences and seminars
to present research results and to interact with other researchers
from other academic and industrial institutions. The Table in the
following pages shows the conference, seminar, or workshop, place
and date, and topics presented by FCRC associated researchers. |
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Table. 1. Conferences, Workshops, and Meetings
Attended by Members of the FCRC. |
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Conference/Seminar/
Workshop
|
Date and Place
|
Attendees/Poster Presenters/Speakers|
* - graduate student;
**- post-doc
***- undergrad student
|
Topic
|
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|
Society for Industrial
Microbiology |
Minneapolis, Minnesota
August 10-14, 2003
|
Bryce Causey*
(Speaker) |
Engineering The Metabolism Of
Escherichia coli W3110 For The Conversion Of Sugar To Oxidized
Products. |
|
|
2nd Symposium on
Bioenergy in Kyoto University 21COE Program |
Kyoto, Japan
October 21-22, 2003
|
Lonnie O. Ingram
(Invited Lecturer) |
Metabolic Engineering Of
Escherichia coli For Fuel Ethanol Production From Biomass |
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American Society for Microbiology
Annual Meeting of the Southeastern and South Carolina Branches |
Athens, Georgia October
30-November 1, 2003 |
Henry C. Aldrich
|
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Bryce Causey*
|
Engineering The Metabolism Of
Escherichia coli W3110 For The Conversion Of Sugar To Oxidized
Products: Acetate Production. |
|
|
Jeremy Purvis* |
Exploiting Trehalose Synthesis For
Improved Osmotic Tolerance in Escherichia coli. |
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|
Brent Wood* |
Improving Klebsiella oxytoca
For Ethanol Production From Lignocellulose. |
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2nd Meeting of S-1007
Multistate Research Committee: The Science and Engineering for
Biobased Industry and Economy |
Washington, DC
November 6-7, 2003
|
Lonnie O. Ingram |
Committee Member |
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5th Recent Advances on
Fermentation Technology (RAFT V) |
St. Petersburg, FL, November 9-11,
2003 |
Shengde Zhou**
(Speaker)
|
Metabolic Engineering
Escherichia coli For Production Of Higher Value Fermentation
Products |
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|
Peer Review Meeting of the Biomass
Program, USDOE, Energy Efficiency and Renewable energy |
Washington, DC
November 18-19, 2003
|
Lonnie O. Ingram
|
Participant |
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Genencor Biomass Fermentation
Group Offsite Meeting |
San Francisco, CA
January 15, 2004
|
Speaker/Participant - Biomass
Fermentation Group Offsite Meeting |
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Interagency Meeting on Metabolic
Engineering |
Washington, DC
February 6, 2004
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Speaker – Re-engineering bacteria
for fuel and chemicals. |
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DOE Genomes to Life Workshop |
Washington, DC
March 3-4, 2004
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Panelist – Roadmapping for Clean
Energy Production. |
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4th Annual IFAS
Graduate Research Symposium |
March 19, 2004 |
Christopher J. Rueter*
Malgorzata A. Gil*
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Presenter – “Pass the Salt Please”
Protein Degradation in Halophiles
Poster – From the Dead Sea to Me –
Probing Proteosome physiology Using Proteomics.
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USDA Citrus & Subtropical Products
Lab |
Winter Haven, FL March 25, 2004 |
Lonnie O. Ingram |
Speaker – Engineering bacteria for
ethanol production. |
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Kosan Biosciences |
Hayward, CA
March 29, 2004
|
Lonnie O. Ingram
|
Speaker and Visit to Industry –
Research Overview |
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Council of Chemical Research |
Tampa, FL
April 17-20, 2004
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Speaker – Re-engineering bacteria
for fuels and chemicals.
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World Congress on Industrial
Biotechnology and Bioprocessing |
Orlando, FL
April 21-23, 2004
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KT Shanmugam |
Speaker – Microbial production of
hydrogen: approaches to enhance yield. - Development of new biofactories.
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The Global Climate and Energy |
Stanford, CA
April 26-27, 2004
|
KT Shanmugam |
Metabolic engineering for fuels
and chemicals. |
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26th Symposium on
Biotechnology for Fuels & Chemicals |
Chattanooga, TN
May 9-12, 2004
|
Brent Wood*
Milind Patel
|
Improving Klebsiella oxytoca
For Ethanol Production From Cellulosic Biomass.
Simultaneous saccharification and
fermentation of lignocellulosic biomass to L(+) lactic acid by a novel
second generation biocatalyst. |
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104TH ASM General
Meeting |
New Orleans, LA
May 23-27, 2004
|
Bryce Causey*
Jeremy Purvis*
Kim Youngyun*
Jonathan Moore |
Engineering the metabolism of
Escherichia coli W3110 for homo-acetate production.
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Fuel Cell Workshop |
Gainesville, FL
May 27-28, 2004
|
LO Ingram
KT Shanmugam
|
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USDA Panel |
Beltsville, MD
June 2-3, 2004
|
LO Ingram |
Interagency Workshop on Metabolic
Engineering |
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DOE Workshop |
Baltimore, MD
June 9, 2004
|
KT Shanmugam |
Hydrogen Production via Direct
Fermentation |
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Service and Awards
Service:
Faculty members have been actively involved in several
academic service activities. They include:
|
Faculty Member |
Type of Service |
Institution/Society |
|
Lonnie O. Ingram |
Fellow |
American Academy of Microbiology,
1998-present; Society of Industrial Microbiology
and Biotechnology, 2001-present |
|
Member
|
U.S. National Academy of Sciences,
2001-present
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Member, Editorial Board |
Biotechnology Letters 1996-present; Metabolic Engineering 1998-present |
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Member, Grant Review Panel
|
DOE, USDA, Interagency Metabolic
Engineering Initiative
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Grant Proposal Reviewer |
NSF, NIH, DOE, USDA, several state
programs, Canada, Australia, Hong Kong, Consortium for Plant
Biotechnology Research |
|
Julie Maupin-Furlow |
Award Committee Chair, 2003-present |
ASM Southeastern Branch |
|
Refereed Journal Reviewer |
Journal of Bacteriology; Archaea,
2001 – present; Frontiers in Bioscience,
2000-present; Molecular Microbiology; Canadian Journal of Microbiology |
|
Outstanding Mentor |
Ronald E. McNair Post Baccalaureate
Achievement Program, UF, 2003 |
|
Member, Editorial Board |
Archives of Biochemistry and
Biophysics; FEMS Letters |
|
James F. Preston, III |
Refereed Journal Reviewer |
Journal of Bacteriology; Science; Applied and Environmental
Microbiology; Journal of Experimental Microbiology; Carbohydrate Research; Physiological and Molecular Plant
Pathology |
|
Member, UF Faculty Advisory Committee |
Vice-President of IFAS, 2002-2004 |
|
Member |
University Senate, 2002-2004 |
|
Graduate Coordinator |
Microbiology and Cell Science
Department. 2003-present |
|
Madeline Rasche |
Secretary-Treasurer |
ASM Southeastern Branch |
|
Refereed Journal Reviewer |
Journal of Bacteriology; Archives of Microbiology
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Grant Proposal Reviewer |
National Science Foundation,
1999-2003 |
Awards:
Outstanding graduate students received travel grants to
present their research results at regional and national meetings, and awards
for best research presentations. Sponsors include: American Society for
Microbiology, Institute of Agricultural and Life Sciences,
and the Davidson Graduate Travel Scholarship.
|
Student |
Award |
Sponsor/Purpose |
|
Jeremy Purvis |
Best Graduate Oral Presentation in
General Microbiology |
ASM Southeastern and South Carolina
Branches Annual Meeting, Athens, GA, October 31-November 13, 2003. |
|
Bryce Causey |
Honorable Mention for Graduate Oral
Presentation in General Microbiology |
|
Jeremy Purvis |
Davidson Graduate Travel Scholarship |
ASM-General Meting, New Orleans, LA
May 23-27, 2004
|
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Bryce Causey
|
IFAS $200 travel Grant |
|
Brent Wood |
26th Symposium on
Biotechnology for Fuels and Chemicals. Chattanooga, TN, May 8-13, 2004 |
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Research Assistantships
Dr. Jimmy G. Cheek, Dean of the College of Agricultural
and Life Sciences, IFAS, granted matching graduate assistantships
to the following students:
|
Graduate Assistant |
Start Date Granted |
Degree |
Mentor |
|
Maegorzata Gil |
2002 |
PhD |
Dr. Julie A. Maupin-Furlow |
|
Jeremy Purvis |
2002 |
MS |
Dr. Lonnie O. Ingram |
|
Franz St. John |
|
PhD |
Dr. James F. Preston, III |
|
Kim Youngyun |
2002 |
PhD |
Dr. K.T. Shanmugam |
|
Phi Min Do |
2003 |
PhD |
Dr. K. T. Shanmugam |
|
Phillip Kirkland |
2003 |
MS |
Dr. Julie A. Maupin-Furlow |
|
Shannon McQuaig |
2003 |
MS |
Dr. Samuel R. Farrah |
|
Jonathan Moore |
2003 |
PhD |
Dr. Lonnie O. Ingram |
|
Amy Wright |
2003 |
MS |
Dr. Julie A. Maupin-Furlow |
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The Center supported 7 other graduate students, 10
post-docs, and 4 research faculty members:
|
Name |
|
Mentor/Lab |
|
|
MS |
Dr. Madeline Rasche |
|
Bryce Causey |
PhD |
Dr. Lonnie O. Ingram |
|
Shai Lawit |
MS |
Dr. William B. Gurley |
|
Steven Kaczowka |
MS |
Dr. Julie A. Maupin-Furlow |
|
Christophen Reuter |
MS |
Dr. Julie A. Maupin-Furlow |
|
Lee Ann Talarico |
PhD |
Dr. Julie A. Maupin-Furlow |
|
Brent Wood |
PhD |
Dr. Lonnie O. Ingram |
|
Patrick Cirino |
Visiting Scientist |
Dr. Lonnie O. Ingram |
|
Yilei Qian |
Research Faculty |
Dr. Lonnie O. Ingram |
|
Shengde Zhou |
Research Faculty |
Dr. Lonnie O. Ingram |
|
Stuart Underwood |
Post-Doc |
Dr. Lonnie O. Ingram |
|
Eun Min Cho |
Post-Doc |
Dr. Nemat Keyhani |
|
Eva Czarnecka-Verner |
Research Faculty |
Dr. William B. Gurley |
|
Haeng Seorg Lee |
Post-Doc |
Dr. William B. Gurley |
|
Kevin O’Grady |
Post-Doc |
Dr. William B. Gurley |
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Tarek Salem |
Post-Doc |
Dr. William B. Gurley |
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Ivanka Karadzic |
Post-Doc |
Dr. Julie Maupin-Furlow |
|
Guang Nong |
Post-Doc |
Dr. James F. Preston, III |
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Liesbeth Schmidt |
Post-Doc |
Dr. James F. Preston, III |
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Virginia Chow |
Post-Doc |
Dr. James F. Preston, III |
|
Tao Han |
Research Faculty |
Dr. K.T. Shanmugam |
|
Milind Patel |
Post-Doc |
Dr. K.T. Shanmugam |
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Patents and Publications
Approved Patents (2):
Application Number
|
Date Approved |
Country |
Authors |
Title |
|
3,457,664 |
01/2004 |
Japan |
Lonnie O. Ingram, David S. Beall,
Gerhard F.H. Burchardt, Walter V. Guimaraes, Kazayosi Ohta, Brent E.
Wood, K. T. Shanmugam |
Ethanol Production by Recombinant
Hosts
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6,569,632 |
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Preston, James F., D.W. Dickson,
John D. Rice, J.H. Charnecki, J.M. Brito, Liesbeth M. Schmidt |
Monoclonal Antibody Probe for
Detection of Adhesins Associated with Mature Endospores of Pasteuria
Spp |
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Pending US Patents (14):
|
Application Number |
Date Submitted |
Authors |
Title |
|
60/424,372 |
11/06/02 |
Lonnie O. Ingram, Thomas B. Causey,
Shengde Zhou, K. T. Shanmugam |
Materials and Methods for the
Efficient Production of Acetate and other Products |
|
10/003,741 |
11/02/02 |
Lonnie O. Ingram, Jacob L. Snoep,
Nico Arfman |
Recombinant Glucose Uptake System |
|
60/418,069 |
10/11/02 |
Lonnie O. Ingram, Shengde Zhou,
Thomas B. Causey, K . T. Shanmugam |
Mutation to Increase Production of
Fermentation Products |
|
60/378,143 |
05/13/02 |
Adnan Hasona, K. T. Shanmugam,
Lonnie O. Ingram |
Novel Pseudomonas putida EstZ
Esterase Gene and Uses Thereof |
|
10/136,960 |
04/30/02 |
Julie A. Maupin-Furlow, Lee Ann
Talarico, Krishnan Chandra Raj, Lonnie O. Ingram |
Cloning and Sequencing of Pyruvate
Decaroxylase (PDC) Genes from Bacteria and Uses Thereof |
|
08/834,900 |
04/16/02 |
Lonnie O. Ingram, Lorraine Yomano,
Sean York |
Development of High-Ethanol
Resistant Escherichia coli |
|
10/001,218 |
11/30/01 |
Lonnie O. Ingram, Maria
Barbosa-Alleyne |
Ethanol Production in Gram-Positive
Microbes |
|
09/885,297 |
06/19/01 |
Shengde Zhou, Lonnie O. Ingram |
Methods and Compositions for
Simultaneous Saccharification and Fermentation |
|
09/885,294 |
06/19/01 |
Lonnie O. Ingram, Stuart Underwood |
Methods for Improving Cell Growth
and Alcohol Production During Fermentation. |
|
60/288,699 |
05/04/01 |
Lonnie O. Ingram, Chanda Raj Krishan,
Julie A. Maupin |
Pyruvate Decarboxylase: A Key Enzyme
for the Oxidative Metabolism of Lactic Acid by
Acetobacter pasteurianus |
|
60/288,698 |
05/04/01 |
Lonnie O. Ingram, Chandra Raj
Krishan, Julie A. Maupin |
|
|
60/288,671 |
05/04/01 |
Lonnie O. Ingram, Lee Ann Talarico,
Julie A. Maupin |
Cloning, Expression, and
Characterization of Pyruvate Decarboxylase fro the Acod Tolerant,
Anaerobic Gram-Positive Bacteriun Sarcinia ventriculi Goodsir |
|
60/288,638 |
05/04/01 |
Lee Ann Talarico, Lonnie O. Ingram,
Julie A. Maupin |
High-level Production of Active
Sarcina ventriculi Pyruvate Decarboxylase in Recombinant
Bacillus megaterium |
|
60/288,622 |
05/04/01 |
Chandra Raj Krishan, Lonnie O.
Ingram, Julie A. Maupin |
Biochemical and Biophysical
characterization of Pyruvate Decarboxylase from the Acetic Acid
Bacterium Acetobacter pastuerianus |
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Pending International Patents (17):
Application
Number
|
Date Submitted |
Country |
Authors |
Title |
|
P 02 01 01630
73450
PCT/US02/15038 |
05/03/02
05/02/02
04/29/02 |
Argentina
Thailand
PCT |
Julie A. Maupin-Furlow, Lee Ann
Talarico, Krishnan Chandra Raj, Lonnie O. Ingram |
Cloning and Sequencing of Pyruvate
Decarboxylase(PDC) Genes from Bacteria and Uses Thereof
|
|
P 01 01 03040
066460
PCT/US01/19690
0946576.4-4-2405-US0119690 |
06/26/01
06/22/01
06/19/01
06/19/01
06/19/01
06/19/01
06/19/01
06/19/01 |
Argentina
Thailand
PCT
Canada
EPO
China
Korea
Vietnam |
Shengde Zhou, Lonnie O. Ingram
|
Methods and Compositions for
Simultaneous Saccharification and Fermentation
|
|
066459
PCT/US01/19641 |
06/22/01
06/19/01 |
Thailand
Argentina
PCT |
Lonnie O. Ingram, Stuart Underwood |
Methods for Improving Cell Growth
and Alcohol Production During Fermentation. |
|
02106753.2
IN/PCT/01/01804/CHE
W-00100102740 |
09/13/02
12/21/01
12/11/01 |
Hongkong
India
Indonesia |
Lonnie O. Ingram, Shengde Zhou |
Recombinant Hosts suitable for
simultaneous Saccharification and Fermentation |
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| Hasona, Adnan, Y. Kim, F.G. Healy,
L.O. Ingram, and K.T. Shanmugam. 2004. Pyruvate formate lyase and acetate
kinase are essential for anaerobic growth of Escherichia coli on
xylose. J. Bacteriol. 186(22): 7593-7600. |
| Patel, M., M. Ou, L.O. Ingram, and
K.T. Shanmugam. 2004. Fermentation of sugarcane bagasse hydrolysate to L(+)
lactic acid by a thermotolerant acidophilic Bacillus sp.
Biotechnology Letters. 26(11):865-8. |
|
Maupin-Furlow JA, Gil MA, Karadzic IM, Kirkland PA, Reuter CJ. 2004.
Proteasomes: perspectives from the Archaea. Front Biosci. 2004 May 1;
9:1743-58. |
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Maupin-Furlow JA, Kaczowka SJ, Reuter CJ, Zuobi-Hasona K, Gil MA. Archaeal
proteasomes: potential in metabolic engineering. Metab Eng. 2003
Jul;5(3):151-63. Review. |
| Underwood, S.A., M.L. Buszko, K.T.
Shanmugam, and L.O. Ingram. 2004. Lack of protective osmolytes limits final
cell density and volumetic productivity of ethanologenic Escherichia
coli KO11 during xylose fermentation. Appl Environ Microbiol. 2004
May;70(5):2734-40. |
| Shukla, V.B., S. Zhou, L.P. Yomano,
K.T. Shanmugam, J.F. Preston, and L.O.Ingram. 2004. Production of
D(-)-lactate from sucrose and molasses. Biotechnology Letters. 26: 689-693. |
| Bobik TA., and Rasche ME. 2004.
Purification and partial characterization of the Pyrococcus horikoshii
methylmalonyl-CoA epimerase. Appl Microbiol Biotechnol. 63:682-685. |
| Rasche ME, Havemann SA, and
Rosentzvaig M. 2004. Characterization of two methanopterin biosynthesis
mutants of Methylobacterium extorquens AM1 by use of a
tetrahydromethanopterin bioassay. J. Bacteriol. 186:1565-1570. |
| Caccamo MA, Malone CS, and Rasche ME.
2004. Biochemical characterization of a dihydromethanopterin reductase
involved in tetrahydromethanopterin biosynthesis in Methylobacterium
extorquens AM1. J. Bacteriol. 186:2068-2073. |
| Uz I, Rasche ME, Townsend T, Ogram
AV, Lindner AS. 2003. Characterization of methanogenic and methanotrophic
assemblages in landfill samples. Proc R Soc Lond B Biol Sci. 270 (Suppl.
2):S202-S205. |
| Schmidt, L.M., J.F. Preston, D.W.
Dickson, J.D. Rice, and T.E. Hewlett. 2003. Environmental quantification of
Pasteuria penetrans endospores using in situ antigen extraction and
immunodetection with a monoclonal antibody. FEMS Mocrobiol. Ecol. 44:17-26 . |
| Preston, J. F., D. W. Dickson, J. E.
Maruniak, G. Nong, J. A. Brito, L. M. Schmidt, and R. M. Giblin-Davis. 2003.
Pasteuria spp. : Systematics and phylogeny of these bacterial
parasites of phytopathogenic nematodes. Journal of Nematology 35:198-207. |
| Preston, J.F., J.C. Hurlbert, J.D.
Rice, A. Ragunathan, and F.J. St. John. 2003. “Microbial Strategies for the
Depolymerization of Glucuronoxylan: Leads to the Biotechnological
Applications of Endoxylanases. ACS Symposium Ser 855, Application of Enzymes
to Lignocellulosics. pp 191-210. |
| JBrito, J.A.,
J. F. Preston, D. W.Dickson, R. M. Giblin-Davis, D. S. Williams, H. C.
Aldrich, and J. D. Rice. 2003.Temporal Production and Immunolocalization of
an Epitope During Pasteuria penetrans sporogenesis. Journal of
Nematology 35(3):278-288. |
| Causey, T.B., K.T. Shanmugam, L.P.
Yomano, and L.O. Ingram. 2003. Engineering Escherichia coli for
efficient conversion of glucose to pyruvate. PNAS. 101(8): 2235-2240 |
| Yilei, Qian, L.P. Yomano, J.F.
Preston, H.C. Aldrich and L.O. Ingram. 2003. Cloning, characterization and
functional expression of the Klebsiella oxytoca xyloside utilization
operon (xynTB) in Escherichia coli. Appl Environ Microbiol.
69(10):5957-67. |
| Schmidt, L. M., J. F. Preston, D. W.
Dickson, J. D. Rice, T. W. Hewlett. 2003. Environmental quantification of
Pasteuria penetrans endospores using in situ antigen extraction and
immunodetection with a monoclonal antibody. FEMS Microbiol. Ecol. 44:17-26. |
| Maupin-Furlow, J.A., S.J. Kaczowka,
C.J. Reuter, K. Zuobi-Hasona, and M.A. Gil. 2003. Archael proteasomes:
potential in metabolic engineering. Metab. Engin. 5:151-163. |
| Kaczowka, S.J. and J.A.
Maupin-Furlow. 2003. Subunit topology of the multiple 20S proteasomes from
Haloferax volcanii. J. Bacteriology 185:165-174 |
| Preston, J. F., D. W. Dickson, J. E.
Maruniak, G. Nong, J. A. Brito, L. M. Schmidt, and R. M. Giblin-Davis. 2003.
Pasteuria spp. : Systematics and phylogeny of these bacterial
parasites of phytopathogenic nematodes. Journal of Nematology 35: 198-207 |
| Brito, J. A, J. F. Preston, D.
W.Dickson, R. M. Giblin-Davis, D. S. Williams, H. C. Aldrich, and J. D.
Rice. 2003. Temporal Production and Immunolocalization of an Epitope During
Pasteuria penetrans sporogenesis. Journal of Nematology 35(3): |
| Bobik, T. A.,
and Rasche, M. E. 2003. HPLC assay for methylmalonyl-CoA epimerase. Anal.
Bioanal. Chem. 375:344-349. |
| Uz, I, Rasche, M.E., Townsend, T.,
Ogram, A. V., and Lindner, A. S. 2003.
Characterization of methanogenic and methanotrophic assemblages in landfill
samples. Proceedings of the Royal Society of London B (Biology Letters
Suppl.) 270:S202-S205. |
| Bechard ME, Chhatwal S, Garcia RE,
Rasche ME. 2003. Application of a colorimetric assay to identify
putative RFAP synthase genes expressed with activity in Escherichia coli.
Biological Procedures Online 5:69-77. |
| Eckert,
Richard B., Henry C. Aldrich, Chris A. Edwards, Bruce A. Cookingham. 2003.
Microscopic differentiation of internal corrosion initiation mechanisms in
natural gas pipeline systems. Corrosion, San Diego, CA |
| Bogan,
Bill W., Wendy R. Sullivan, Kevin J. Kayser, KD Derr, Henry C. Aldrich and
J. Robert Paterek. 2003. Alkanindiges
illinoisensis gen. nov., sp. nov., an obligately hydrocarbonoclastic,
aerobic squalane-degrading bacterium isolated from oilfield soils. Int J
Syst Evol Microbiol 53: 1389-1395. |
| Gonzalez, R., H. Tao, J. E. Purvis,
S. W. York, K. T. Shanmugam and L. O. Ingram.2003. Gene array-based
identification of beneficial changes that contribute to increased ethanol
tolerance in ethanologenic Escherichia coli LY01. Biotechnol. Prog.
19:612-623. |
| Zhou, Shengde, K.T. Shanmugam and L.O.
Ingram. 2003. Functional replacement of Escherichia coli D(-)-lactate
dehydrogenase (ldhA) with L(+) -lactate dehydrogenase (ldhL)
from Pediococcus acidilactici. Appl. Environ. Microbiol. 60(4):
2237-2244. |
|
Causey, T. B., S. Zhou, K. T. Shanmugam, and L. O. Ingram. 2003. Engineering
the metabolism of Escherichia coli W3110 for the conversion of sugar
to redox-neutral and oxidized products: homo-acetate production. Proc. Natl.
Acad. Sci. USA. 100:825-832.
|
| Zhou, S., T.B.
Causey, A. Hasona, K.T. Shanmugam, and L.O. Ingram. 2003. Production of
optically pure D-lactic acid in mineral salts medium by metabolically
engineered Escherichia coli W3110. Appl. Environ. Microbiol.
69(1):399-407. |
|
Giblin-Davis, R.M., D. S. Williams, S. Bekal, D. W. Dickson,
J. A. Brito, J. O. Becker and J. F. Preston. 2003
'Candidatus Pasteuria usgae' sp. nov.,
an obligate endoparasite of the phytoparasitic nematode Belonolaimus
longicaudatus. Int J Syst Evol Microbiol 53: 197-200. |
|
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On-Going Research
Projects
1. Utilization of hemicellulose-derived
glucurunoxylose for ethano.l production.
Investigator:
James F. Preston, III.,
Lonnie O. Ingram
Sponsor:
Consortium for Plant Biotechology Research
(CPBR)
Objective:
The goal of this research is to develop biocatalysts for the cost-effective
production of alternative fuels and biobased
products from the underutilized sourced of renewable biomass.
Duration:
07/01/2002 – 06/30/2004
Amount:
$58,890
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2. Physiology of proteosomes in Haloferax
volcanii
Investigator:
Julie A. Maupin-Furlow
Sponsor:
NIH National Institute of
Health
Objective:
Duration: 04/01/2000 – 03/31/2005
Amount: $888,840
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3. Methanopterin biosynthesis in archaea and
methylotropic bacteria
Investigator:
Madeline E. Rasche
Sponsor:
NSF National Science
Foundation
Objective: Methanopterin is an analog of folate that was originally
discovered in methane-producing microorganisms.
The overall goal of the methanopterin research project is to use a combined
biochemical and genetics
approach to investigate the molecular basis of methanopterin biosynthesis in
archaea and methylotrophic bacteria.
Duration: 05/01/1999 – 11/30/2004
Amount:
$493,000
|
|
4. Advanced separation processes
Investigator:
Samuel R. Farrah
Sponsor:
NSF National Science
Foundation
Duration: 09/15/1994 – 09/30/2003
Amount:
$174,293
|
|
5. Surface modification for advancing response
processes and environmental protection
Investigator:
Samuel R. Farrah
Sponsor:
NSF National Science Foundation
Duration: 09/01/2002 – 08/31/2005
Amount: $25,000
|
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6. Virus monitoring of effluent from joint
facility
Investigator:
Samuel R. Farrah
Sponsor: Orange County, Florida
Duration: 10/01/2002 – 09/30/2003
Amount: $29,550
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7. Application of functional genomics to
recombinant biocatalysts for fuels and chemicals
Investigators:
Lonnie O. Ingram,
K.T.Shanmugam,
James F. Preston,
Julie A. Maupin-Furlow,
Henry C. Aldrich,
Marian Buszko, Jon
Stewart,
Nancy D. Denslow,
Atul Narang
Sponsor:
U.S. Department of Agriculture/Coop
SRS
Duration: 09/15/2000 – 09/30/2003
Amount: $2,000,000
|
|
8. Engineering bacteria for fuel ethanol
production.
Investigator:
Lonnie O. Ingram
Sponsor:
U.S. Department of Agriculture/Coop
SRS
Duration: 09/01/2001 – 08/31/2004
Amount: $300,000
|
|
9.
The genome of Pasteuria penetrans: a blueprint for developiing
pasteuria spp. for biocontrol of plant paratistic nematode
Investigators:
James F. Preston,III,
DW Dickson
Sponsor:
U.S. Department of Agriculture
Objective:
Duration: 09/15/2002 – 09/14/2004
Amount: $80,813
|
|
10. Sentinel plants utilizing bacterial
two-component signaling pathways
Investigators:
Lonnie O. Ingram, William.B.
Gurley,
Thomas A. Bobik,
Nemat O. Keyhani,
Shouguang Jin
Sponsor:
U.S. Department of Defense,
DARPA (*Defense Advanced
Research Projects Agency)
Objective: Transfer a functional bacterial two-component bacterial
system into Arabidopsis. The bacterial system
will be engineered to detect a variety of analytes. In plats, the system
will drive a variety of reporter genes.
Duration: 06/01/2002 – 05/31/2005
Amount: $2,361,230
|
|
11. Ethanol tolerant biocatalysts for fuel
ethanol production./Genetic improvement of Escherichia coli
for fuel ethanol production.
Investigator:
Lonnie O. Ingram
Sponsor:
U.S. Department of Energy
Objective: The objective of this continuation research is to develop
improved biocatalysts which simplify the
bioethanol process using gene array analysis and microbial physiology to
guide genetic improvements.
Duration: 07/01/1996 – 06/30/2005
Amount: $1,300,000
|
|
12. Complementation of endoxylanase with glucoronidase
and xylosidase for bacterial production of ethanol
from lignocellulosic biomass
Investigators:
James F. Preston, III,
Lonnie O. Ingram,
K.T. Shanmugam
Sponsor:
U. S. Department of Energy
Objective: The general objective is to quantitatively convert all of
the sugars in the hemicellulose fraction
of plant biomass to ethanol.
Duration: 09/01/2000 – 08/31/2004
Amount: $439,061
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|
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The Center would like to acknowledge
all its supporters and sponsors:
UF/IFAS:
Dr. Michael V. Martin, Vice President for Agriculture and Natural Resources
Dr. Richard L. Jones, Dean for Research and
FAES
Dr. Jimmy Cheek, Dean for Academic Programs and the College of Agriculture
and Life Sciences
Department of Microbiology and Cell Science
Grant Agencies:
Biotechnology and Research Development Corporation (BRDC)
Consortium for Plant Biotechnology, Inc. (CPBR)
Defense Advanced Research Project Agency (DARPA/USDD)
National Institutes of Health
National Science Foundation
Orange County, Florida
US Department of Agriculture
US Department of Energy
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